ZNF658 Polyclonal antibody

ZNF658 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24823-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Zinc finger protein 658

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of fetal human brain using 24823-1-AP
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 24823-1-AP (ZNF658 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 24823-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24823-1-AP (ZNF658 Antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 24823-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24823-1-AP (ZNF658 Antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications
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Tested Applications

Positive WB detected in	fetal human brain tissue
Positive IHC detected in	human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24823-1-AP targets ZNF658 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag20508 Product name: Recombinant human ZNF658 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 71-245 aa of BC031626 Sequence: FLNQRYPGYFKVDHIKGIREKQEKPLWQEIFISDADKTLSKEGQKVLEKPFNLEIAPELSEKISCKCDSHRMNLPVASQLIISERKYSRKKTEYMNVCEKLQLDIKHEKAHAEEKSYEHGENAKAFSYKKDQHWKFQTLEESFECDGSGQGLYDKTICITPQSFLTGEKSCKDDE Predict reactive species
Full Name	zinc finger protein 658
Calculated Molecular Weight	1059 aa, 122 kDa
Observed Molecular Weight	71 kDa
GenBank Accession Number	BC031626
Gene Symbol	ZNF658
Gene ID (NCBI)	26149
RRID	AB_2879744
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5TYW1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF658 (Zinc Finger Protein 658), also known as DKFZp572C163 or FLJ32813, is a Krüppel C2H2-type zinc-finger transcription factor that binds cis-regulatory DNA and represses transcription. ZNF658 is a master regulator of zinc homeostasis and ribosome biogenesis.