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  • Featured Product
  • KD/KO Validated

ZNF671 Polyclonal antibody

ZNF671 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 21329-1-AP

Synonyms

zinc finger protein 671, ZNF671

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ZNF671 Polyclonal antibody (21329-1-AP)

    WB analysis using 21329-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21329-1-AP (ZNF671 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ZNF671 Polyclonal antibody (21329-1-AP)

    WB analysis of HepG2 using 21329-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21329-1-AP (ZNF671 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using ZNF671 Polyclonal antibody (21329-1-AP)

    IF Staining of U-251 using 21329-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using ZNF671 antibody (21329-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, HepG2 cells, HeLa cells, MCF-7 cells, MDA-MB-231 cells, SKOV-3 cells, U-251 cells
Positive IF detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21329-1-AP targets ZNF671 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF671 fusion protein Ag15319
Full Name zinc finger protein 671
Calculated Molecular Weight 556 aa, 63 kDa
Observed Molecular Weight 61-70 kDa
GenBank Accession NumberBC025728
Gene Symbol ZNF671
Gene ID (NCBI) 79891
RRIDAB_10755298
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZNF671 antibody 21329-1-APDownload protocol
IF protocol for ZNF671 antibody 21329-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

The Tumor Suppressor Role of Zinc Finger Protein 671 (ZNF671) in Multiple Tumors Based on Cancer Single-Cell Sequencing.

Authors - Jian Zhang
View Article
humanWB

Front Oncol

Epigenetic-Mediated Downregulation of Zinc Finger Protein 671 (ZNF671) Predicts Poor Prognosis in Multiple Solid Tumors.

Authors - Jian Zhang
View Article
humanWB

J Exp Clin Cancer Res

Epigenetic mediated zinc finger protein 671 downregulation promotes cell proliferation and tumorigenicity in nasopharyngeal carcinoma by inhibiting cell cycle arrest.

Authors - Jian Zhang
  • KD Validated
View Article
humanWB

Int J Biol Sci

Increased methylation of ZNF671 suppresses tumor progression by promoting MAPK6 transcription in laryngeal carcinoma

Authors - Wei Zhang
View Article