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  • KD/KO Validated

ZRANB3 Polyclonal antibody

ZRANB3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 23111-1-AP

Synonyms

ZRANB3



Tested Applications

Positive WB detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23111-1-AP targets ZRANB3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZRANB3 fusion protein Ag19407
Full Name zinc finger, RAN-binding domain containing 3
Calculated Molecular Weight 1079 aa, 123 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC064616
Gene Symbol ZRANB3
Gene ID (NCBI) 84083
RRIDAB_2744527
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZRANB3, also named Annealing helicase 2 or DNA annealing helicase and endonuclease ZRANB3, is a 1079 amino acid protein, which contains one RanBP2-type zinc finger, one helicase C-terminal domain, one HNH domain and one helicase ATP-binding domain. ZRANB3 localizes in the nucleus and belongs to the SNF2/RAD54 helicase family. ZRANB3 as a DNA annealing helicase and endonuclease is required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events.

Protocols

Product Specific Protocols
WB protocol for ZRANB3 antibody 23111-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Excessive reactive oxygen species induce transcription-dependent replication stress

Authors - Martin Andrs
  • KD Validated
humanWB

Mol Cell

Active DNA damage eviction by HLTF stimulates nucleotide excision repair.

Authors - Marvin van Toorn
  • KD Validated
humanWB

Mol Cell

Replication Fork Slowing and Reversal upon DNA Damage Require PCNA Polyubiquitination and ZRANB3 DNA Translocase Activity.

Authors - Marko Vujanovic
  • KD Validated
humanWB

Mol Cell

Fork Cleavage-Religation Cycle and Active Transcription Mediate Replication Restart after Fork Stalling at Co-transcriptional R-Loops.

Authors - Nagaraja Chappidi
  • KD Validated
humanWB

Mol Cell

CtIP-Mediated Fork Protection Synergizes with BRCA1 to Suppress Genomic Instability upon DNA Replication Stress.

Authors - Sara Przetocka
  • KD Validated
humanWB

Nat Commun

Replication fork reversal triggers fork degradation in BRCA2-defective cells.

Authors - Sofija Mijic
  • KO Validated