Tested Applications
| Positive WB detected in | PC-3 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14281-1-AP targets ZWILCH in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5454 Product name: Recombinant human ZWILCH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 179-477 aa of BC036900 Sequence: KSAVELVQEFLNDLNKLDGFGDSTKKDTEVETLKHDTAAVDRSVKRLFKVRSDLDFAEQLWCKMSSSVISYQDLVKCFTLIIQSLQRGDIQPWLHSGSNSLLSKLIHQSYHGTMDTVSLSGTIPVQMLLEIGLDKLKKDYISFFIGQELASLNHLEYFIAPSVDIQEQVYRVQKLHHILEILVSCMPFIKSQHELLFSLTQICIKYYKQNPLDEQHIFQLPVRPTAVKNLYQSEKPQKWRVEIYSGQKKIKTVWQLSDSSPIDHLNFHKPDFSELTLNGSLEERIFFTNMVTCSQVHFK Predict reactive species |
| Full Name | Zwilch, kinetochore associated, homolog (Drosophila) |
| Calculated Molecular Weight | 67 kDa |
| Observed Molecular Weight | ~60 kDa |
| GenBank Accession Number | BC036900 |
| Gene Symbol | ZWILCH |
| Gene ID (NCBI) | 55055 |
| RRID | AB_2878040 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H900 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZWILCH is a new component of the ZW10/ROD complex. The complex is a key player in the spindle checkpoint. ZWILCH has two isoforms with MW 67 kDa and 54 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZWILCH antibody 14281-1-AP | Download protocol |
| WB protocol for ZWILCH antibody 14281-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZWILCH Polyclonal antibody (Cat# 14281-1-AP) has 2 citations. These appear in Nucleic Acids Research and Biomedicines. The cited research spans cell biology (spindle assembly checkpoint regulation) and oncology (adrenocortical carcinoma prognosis).
| Species | Application | Title |
|---|---|---|
Biomedicines The Enhanced Expression of ZWILCH Predicts Poor Survival of Adrenocortical Carcinoma Patients |
