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Tested Applications
| Positive WB detected in | human kidney tissue, A431 cells, HEK-293 cells, mouse kidney tissue |
| Positive IF/ICC detected in | Hela cells |
ZXDC has low expression in most cells and tissues
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
20530-1-AP targets ZXDC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14392 Product name: Recombinant human ZXDC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 173-522 aa of BC002940 Sequence: MDLAALFSDTPANASGSAGGSDEALNSGILTIDVTSVSSSLGGNLPANNSSLGPMEPLVLVAHSDIPPSLDSPLVLGTAATVLQQGSFSVDDVQTVSAGALGCLVALPMKNLSDDPLALTSNSNLAAHITTPTSSSTPRENASVPELLAPIKVEPDSPSRPGAVGQQEGSHGLPQSTLPSPAEQHGAQDTELSAGTGNFYLESGGSARTDYRAIQLAKEKKQRGAGSNAGASQSTQRKIKEGKMSPPHFHASQNSWLCGSLVVPSGGRPGPAPAAGVQCGAQGVQVQLVQDDPSGEGVLPSARGPATFLPFLTVDLPVYVLQEVLPSSGGPAGPEATQFPGSTINLQDLQ Predict reactive species |
| Full Name | ZXD family zinc finger C |
| Calculated Molecular Weight | 858 aa, 90 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC002940 |
| Gene Symbol | ZXDC |
| Gene ID (NCBI) | 79364 |
| RRID | AB_10694823 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q2QGD7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ZXDC antibody 20530-1-AP | Download protocol |
| WB protocol for ZXDC antibody 20530-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Cell-fate transition and determination analysis of mouse male germ cells throughout development. | ||
iScience ZXDC enhances cervical cancer metastasis through IGF2BP3-mediated activation of RhoA/ROCK signaling | ||
Cancer Cell The crosstalk between macrophages and cancer cells potentiates pancreatic cancer cachexia |
