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p70(S6K) Polyclonal antibody

p70(S6K) Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14485-1-AP

Synonyms

p70 alpha, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K 1, p70 S6K alpha, p70 S6KA, p70(S6K), p70(S6K) alpha, p70S6K, P70S6K1, PS6K, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K beta 1, S6K1, STK14A



Tested Applications

Positive WB detected inHEK-293 cells, IGF-1 treated MCF-7 cells, Jurkat cells, NIH/3T3 cells, HeLa cells, PC-12 cells, C6 cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14485-1-AP targets p70(S6K) in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit, pig, Megalobrama amblycephala
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen p70(S6K) fusion protein Ag5883
Full Name ribosomal protein S6 kinase, 70kDa, polypeptide 1
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 65-75 kDa, 80-85 kDa
GenBank Accession NumberBC053365
Gene Symbol RPS6KB1
Gene ID (NCBI) 6198
RRIDAB_2269787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA, and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of mTOR and acts as a crucial effector of mTOR signaling pathway. It plays a key role in cell growth and proliferation by regulating INS sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy (PMID:22684641). The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K). The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation, and metabolism and is the central regulator of translation of some components of the protein synthesis system. Due to alternative translation, two isoform S6K1 proteins are known to exist in mammalian cells: p85 S6K1 and p70 S6K1, which is identical to p85 S6K but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1).

Protocols

Product Specific Protocols
WB protocol for p70(S6K) antibody 14485-1-APDownload protocol
IHC protocol for p70(S6K) antibody 14485-1-APDownload protocol
IF protocol for p70(S6K) antibody 14485-1-APDownload protocol
IP protocol for p70(S6K) antibody 14485-1-APDownload protocol
FC protocol for p70(S6K) antibody 14485-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Nature

Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.

Authors - Xiao-Yi Lu
mouseWB

Cell Stem Cell

Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis.

Authors - Changzheng Li
mouseIF

Small Methods

PROTAC Degraders of Androgen Receptor-Integrated Dissolving Microneedles for Androgenetic Alopecia and Recrudescence Treatment via Single Topical Administration

Authors - Ruxuan Wang
humanWB

Cell Death Differ

Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration

Authors - Huaizhen Liang
human,mouseWB

Redox Biol

Aberrant hyper-expression of the RNA binding protein GIGYF2 in endothelial cells modulates vascular aging and function

Authors - Fanglin Niu
mouseWB

Autophagy

SMCR8 negatively regulates AKT and MTORC1 signaling to modulate lysosome biogenesis and tissue homeostasis.

Authors - Yungang Lan