phospho-CHC1(Ser12) Antibody
Rabbit Polyclonal
Catalog number: 14444-1-AP
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Applications
| Tested applications | ELISA, WB, IHC |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells |
| Positive IHC detected in | human ovary tumor |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:20-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
-Antibody-14444-1-AP-p174597wb.jpg "HeLa cells were subjected to SDS PAGE followed by western blot with 14444-1-AP(phospho-CHC1(Ser12) Antibody) at dilution of 1:600")
HeLa cells were subjected to SDS PAGE followed by western blot with 14444-1-AP(phospho-CHC1(Ser12) Antibody) at dilution of 1:600-Antibody-14444-1-AP-IHC3328.jpg "Immunohistochemistry of paraffin-embedded human ovary tumor using 14444-1-AP(phospho-CHC1(Ser12) Antibody) at Dilution 1:50 (under 10x lens)")
Immunohistochemistry of paraffin-embedded human ovary tumor using 14444-1-AP(phospho-CHC1(Ser12) Antibody) at Dilution 1:50 (under 10x lens)-Antibody-14444-1-AP-IHC3329.jpg "Immunohistochemistry of paraffin-embedded human ovary tumor using 14444-1-AP(phospho-CHC1(Ser12) Antibody) at Dilution 1:50 (under 40x lens)")
Immunohistochemistry of paraffin-embedded human ovary tumor using 14444-1-AP(phospho-CHC1(Ser12) Antibody) at Dilution 1:50 (under 40x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | Peptide | Full name | regulator of chromosome condensation 1 |
|
Calculated molecular weight |
48kd |
Observed molecular
weight |
45-50kd |
|
GenBank accession number |
NM_001048194 | Gene ID (NCBI) | 1104 |
| Gene symbol | RCC1 | Synonyms | CHC1; RCC1-I |
Background
CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against human phospho-CHC1(Ser12) antigen. |
