phospho-Marcks Antibody
Rabbit Polyclonal
Catalog number: 10018-3-AP
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Applications
| Tested applications | ELISA, WB |
| Cited applications | WB |
| Species specificity | human, mouse; other species not tested. |
| Cited species | bovine, human, rat |
| Positive WB detected in | Mouse J774 Macrophage cellss-treated with PMA |
| Recommended dilution | WB: 1:500-1:5000 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
Mouse J774 Macrophage cellss-treated with PMA were subjected to SDS PAGE followed by western blot with 10018-3-AP(phospho-Marcks Antibody) at dilution of 1:1500
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | Recombinant Protein | Full name | myristoylated alanine rich protein kinase C substrate |
|
Calculated molecular weight |
32kd |
Observed molecular
weight |
|
|
GenBank accession number |
BC046601 | Gene ID (NCBI) | 17118 |
| Gene symbol | Marcks | Synonyms | Macs; PKCSL |
Background
MARCKS is a member of MARCKS family of PKC substrate and it is also a widely accepted indicator of PKC activation. It shows a ubiquitous tissue distribution. The calculated molecular weight of MARCKS is 39 kDa and its apparent molecular weights were found at 68 kDa to 90 kDa in different species. This protein is a known to regulated cytoskeleton remodeling and membrane recycling. The anti-MARCKS antibody is raised against purified MARCKS protein from mouse brain using a method similar to that previously reported for MacMARCKS |
