Tested Applications
| Positive WB detected in | human placenta tissue, mouse liver tissue, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18507-1-AP targets PLAP in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13361 Product name: Recombinant human ALPP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 199-535 aa of BC009647 Sequence: SARQEGCQDIATQLISNMDIDVILGGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTDAAHPGRSVVPALLPLLAGTLLLLETATAP Predict reactive species |
| Full Name | alkaline phosphatase, placental (Regan isozyme) |
| Calculated Molecular Weight | 58 kDa |
| Observed Molecular Weight | 66 kDa |
| GenBank Accession Number | BC009647 |
| Gene Symbol | PLAP |
| Gene ID (NCBI) | 250 |
| RRID | AB_10644334 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05187 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PLAP antibody 18507-1-AP | Download protocol |
| IP protocol for PLAP antibody 18507-1-AP | Download protocol |
| WB protocol for PLAP antibody 18507-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PLAP Polyclonal antibody (catalog #18507-1-AP) has 21 citations. These appear in journals including J Nanobiotechnology, Advanced Science, Biomaterials, Phytomedicine, Materials Today Bio, Acta Biomaterialia, International Journal of Biological Macromolecules, Progress in Orthodontics, ACS Biomaterials Science & Engineering, Aging, Journal of Dentistry, Frontiers in Endocrinology, International Immunopharmacology, Immunotargets and Therapy, Bone, Journal of Orthopaedic Surgery and Research, Molecular and Cellular Endocrinology, Amino Acids, and Oral Diseases. The research fields span bone biology, osteoporosis, osteogenesis, periodontal regeneration, bone healing, and related biomaterials and immunology studies.
| Species | Application | Title |
|---|---|---|
J Nanobiotechnology Ultrasound-triggered piezoelectric polyetheretherketone with boosted osteogenesis via regulating Akt/GSK3β/β-catenin pathway | ||
J Nanobiotechnology Nanoparticle-integrated smart hydrogel with H(2)S-triggered antibacterial escalation and sustained oxygen release for periodontal regeneration. | ||
Biomaterials NRF2 is a critical regulator and therapeutic target of metal implant particle-incurred bone damage | ||
Phytomedicine Bushentongluo formula counteracts BMSCs senescence to alleviate postmenopausal osteoporosis by activating mitophagy via the SENP1-SIRT3 pathway. | ||
Mater Today Bio Engineered bone-targeting Lycium barbarum L.-derived extracellular vesicle-like nanoparticles deliver miR167a-5p to regulate mitochondrial dynamics and metabolic reprogramming in alleviating osteoporosis. |
