Tested Applications
| Positive WB detected in | HEK-293 cells, rat kidney tissue, human stomach tissue, mouse ovary tissue, mouse colon tissue, mouse kidney tissue, U2OS cells |
| Positive IP detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16961-1-AP targets CA2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10680 Product name: Recombinant human CA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-260 aa of BC011949 Sequence: MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDTHTAKYDPSLKPLSVSYDQATSLRILNNGHAFNVEFDDSQDKAVLKGGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFAARGLLPESLDYWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASFK Predict reactive species |
| Full Name | carbonic anhydrase II |
| Calculated Molecular Weight | 260 aa, 29 kDa |
| Observed Molecular Weight | 29 kDa |
| GenBank Accession Number | BC011949 |
| Gene Symbol | CA2 |
| Gene ID (NCBI) | 760 |
| RRID | AB_2065857 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P00918 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CA2(carbonic anhydrase II ) is also called Car2, CA-II, and CAII and belongs to the alpha-carbonic anhydrase family. It reversibly hydrates CO2 in cellular ion transport and homeostasis. CA2 is essential for bone resorption and osteoclast differentiation. It regulates SLC9A1 activity via a phosphorylation-regulated CAII binding site in SLC9A1 tail(PMID:16475831). It also may be involved in brain development (PMID:16825953). Defects in CA2 cause osteopetrosis autosomal recessive type 3 (OPTB3).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for CA2 antibody 16961-1-AP | Download protocol |
| WB protocol for CA2 antibody 16961-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CA2 Polyclonal antibody (Cat# 16961-1-AP) has 18 citations spanning journals including Cell Reports, Phytomedicine, Journal of Medicinal Chemistry, Journal of Ethnopharmacology, Molecular Cell Proteomics, Scientific Reports, PNAS Nexus, Journal of Physiology, and Frontiers in Molecular Neuroscience. Research fields cover oncology (breast, liver, cervical, ovarian, nasopharyngeal, and colon cancers), ethnopharmacology, neuroscience, bone biology, renal fibrosis, vascular calcification, and metabolic regulation.
| Species | Application | Title |
|---|---|---|
Phytomedicine Sanhua essential oil exerts antidepressant effects in breast cancer-related depression by modulating metabolic pathways. | ||
J Genet Genomics The single cell transcriptomic landscape of recurrent giant cell tumor of bone following neoadjuvant denosumab therapy. | ||
Cell Rep Carbonic anhydrase 2 facilitates sorafenib resistance by counteracting MCT4-mediated intracellular pH dysregulation in HCC | ||
Cell Rep Hi-C profiling in tissues reveals 3D chromatin-regulated breast tumor heterogeneity informing a looping-mediated therapeutic avenue | ||
J Med Chem Intracellular Binding of Novel Fluorinated Compounds to Carbonic Anhydrase Isoforms Explored by In-Cell (19)F NMR. | ||
J Ethnopharmacol TCM compatibility-driven mechanism of Shenshuaining (SSN) capsules against renal fibrosis: Integrated metabolomic and network analyses. |
Reviews
What customers say
Both reviewers rated this antibody 5 stars for Western Blot applications. One user reported a strong signal at a 1:1000 dilution (overnight at 4°C) in MCF7 cells. The other used it at 1:500 to detect carbonic anhydrase II in primary mouse osteoclasts, noting a very clean blot with no non-specific bands. Overall, users highlight strong signal intensity and high specificity across different cell types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Adrianna (Verified Customer) (10-31-2022) | Very good antibody, 1:1000 dilution incubated ON at 4 deg C gave a strong signal.
|
Isabel (Verified Customer) (07-03-2020) | Antibody was used to detect carbonic anhydrase II in protein samples taken from primary mouse osteoclasts. The blot produced was really clean with no other bands.
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