TBP Antibody 1 Publications

Mouse Monoclonal| Catalog number: 66166-1-Ig |CloneNo.: 2H3B2

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Freight/Packing

Con: 225 μg/150 μl

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Species specificity:
human, mouse, rat, pig

Positive WB detected in:
HEK-293 cells, pig liver tissue

Positive IP detected in:
HEK-293 cells

Positive IHC detected in:
human breast cancer tissue, human breast cancer tissue, human liver cancer tissue

Positive IF detected in:
HeLa cells

Recommended dilution:
WB : 1:2000-1:16000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500

Product Information


Source:
Mouse

Purification method:
Protein A purification

Isotype:
IgG2a

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
TATA box binding protein

Calculated molecular weight:
338aa,38 kDa

Observed molecular weight:
mouse/rat 33-36 kDa and human 37-43kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
TBP

Synonyms
GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID
Background

The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).


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