1X10^6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2) and 0.5 ug FITC Plus Anti-Mouse CD4 (FITC-65104, Clone: GK1.5) or 0.5 ug FITC Plus rat IgG2b isotype control. Cells were not fixed.

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1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 and 0.2 ug PE Anti-Mouse CD4 (PE-65104, Clone:GK1.5) or 0.2 ug Isotype Control. Cells were not fixed.

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1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 488 Anti-Mouse CD3 and 0.2 ug APC Anti-Mouse CD4 (APC-65104, Clone: GK1.5) or 0.2 ug Isotype Control. Cells were not fixed.

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1x10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD4 (GK1.5) (65104-1-Ig, Clone:GK1.5) or 0.5 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2) and FITC anti-rat IgG2b Antibody, and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.

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1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD4 (CL647-65104, Clone: GK1.5) or CoraLite® Plus 647-Conjugated rat IgG2b isotype control. Cells were not fixed.

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Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD4 (CL647-65104, Clone: GK1.5) at dilution of 1:500.

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1X10^6 mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 405 Anti-Mouse CD4 (CL405-65104, Clone:GK1.5) or 0.5 ug Control Antibody. Cells were not fixed.

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1X10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD4 (CL488-65104, Clone:GK1.5) or 0.25 ug CoraLite® Plus 488 Rat IgG2b Isotype Control (LTF-2) (CL488-65211, Clone: LTF-2), and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.

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Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD4 (CL488-65104, Clone: GK1.5) at dilution of 1:500.

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1X10^6 mouse splenocytes were surface co-stained with APC Anti-Mouse CD3 and 0.5 ug CoraLite® Plus 555 Anti-Mouse CD4 (CL555-65104, Clone: GK1.5) or 0.5 ug Isotype Control. Cells were not fixed.

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