WB result of PSD95-Specific,DLG4 antibody (20665-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSD95-Specific,DLG4 transfected HeLa cells.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20665-1-AP (PSD95-Specific,DLG4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20665-1-AP (PSD95-Specific,DLG4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Various lysates were subjected to SDS PAGE followed by western blot with 20665-1-AP (PSD95-Specific,DLG4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human PSD95-Specific,DLG4 (20665-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).