Various lysates were subjected to SDS PAGE followed by western blot with 11565-1-AP (STIM1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11565-1-AP (STIM1 antibody) at dilution of 1:50 (under 40x lens).
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using STIM1 antibody (11565-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human STIM1 (11565-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).