Various lysates were subjected to SDS PAGE followed by western blot with 24613-1-AP (Angiopoietin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24613-1-AP (Angiopoietin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24613-1-AP (Angiopoietin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Angiopoietin 2 antibody (24613-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using Angiopoietin 2 antibody (24613-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).