Various lysates were subjected to SDS PAGE followed by western blot with 16001-1-AP (ARG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 16001-1-AP (ARG1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours; mouse liver tissue and rat liver tissue are positive, other cells express no or low ARG1 under normal condition.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16001-1-AP (Arginase-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP result of anti-Arginase-1 (IP:16001-1-AP, 4ug; Detection:16001-1-AP 1:700) with mouse liver tissue lysate 5200ug.
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Arginase-1 antibody (16001-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Arginase-1 antibody (16001-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).