1x10^6 HeLa cells were stained with 0.25 ug Anti-Human N-cadherin (98006-1-RR, Clone: 230259D7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Control Antibody and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (blue). Cells were not fixed.

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1x10^6 human monocyte-derived immature dendritic cells were surface stained with 0.25 ug Anti-Human B7-H3/CD276 Rabbit Recombinant Antibody (98017-1-RR, Clone: 230305A10) and PE-conjugated Goat Anti-Rabbit IgG (red), or 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG (blue). Cells were not fixed.

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1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 (98007-1-RR, Clone:230268E6) or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then co-stained with APC Anti-Human CD19 (HIB19) (APC-65110, Clone: HIB19). Cells were not fixed. Lymphocytes were gated.

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Surface Plasmon Resonance (SPR) kinetic assays of 98007-1-RR against Human CD200 were performed. The affinity constant is 2.687 nM.

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1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 (98007-2-RR, Clone:230268E6) or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Human CD19 (HIB19) (APC-65110, Clone: HIB19). Cells were not fixed. Lymphocytes were gated.

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1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 (98007-2-PBS, Clone:230268E6) or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Human CD19 (HIB19) (APC-65110, Clone: HIB19). Cells were not fixed. Lymphocytes were gated.

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1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 (98007-1-PBS, Clone:230268E6) or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then co-stained with APC Anti-Human CD19 (HIB19) (APC-65110, Clone: HIB19). Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD226 Rabbit Recombinant Antibody (98002-1-RR, Clone:41A3) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD226 Rabbit Recombinant Antibody (98002-1-RR, Clone:41A3) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

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Biolayer interferometry (BLl) kinetic assays of 98002-1-RR against Human CD226 were performed. The affinity constant is 37.1 nM.

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1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD226 Rabbit Recombinant Antibody (98002-1-PBS, Clone:41A3) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD226 Rabbit Recombinant Antibody (98002-1-PBS, Clone:41A3) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

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