Western Blot (WB) guide and troubleshooting tips.
From sample preparation to tackling issues such as non-specific bands, the complete guide to western blotting by Proteintech offers a comprehensive step-by-step protocol and solutions to the most common WB problems experienced in the lab. Get your WB protocol guide by filling in the form on the right.
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Simple step-by-step western blot protocol
Reduce poor signal and nonspecific bands
Find easy to follow SDS-PAGE gel recipes
Choose the right lysis buffer for your protein
Get specific bands with low MW proteins