HeLa cells were subjected to SDS PAGE followed by western blot with 14503-1-AP( 14-3-3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

human brain tissue were subjected to SDS PAGE followed by western blot with 14503-1-AP(14-3-3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 14503-1-AP(14-3-3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 14503-1-AP( 14-3-3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

mouse heart tissue were subjected to SDS PAGE followed by western blot with 14503-1-AP( 14-3-3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14503-1-AP(14-3-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse liver tissue were subjected to SDS PAGE followed by western blot with 14503-1-AP(14-3-3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse liver tissue were subjected to SDS PAGE followed by western blot with 14503-1-AP( 14-3-3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

mouse lung tissue were subjected to SDS PAGE followed by western blot with 14503-1-AP(14-3-3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Multiple lysates were subjected to SDS PAGE followed by western blot with 14503-1-AP( 14-3-3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

rat liver tissue were subjected to SDS PAGE followed by western blot with 14503-1-AP( 14-3-3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Immunohistochemistry of paraffin-embedded human testis tissue slide using 14503-1-AP( 14-3-3 Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human testis tissue slide using 14503-1-AP( 14-3-3 Antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 14503-1-AP( 14-3-3 Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 14503-1-AP( 14-3-3 Antibody) at dilution of 1:50 (under 40x lens)

IP Result of anti-14-3-3 (IP:14503-1-AP, 5ug; Detection:14503-1-AP 1:500) with mouse lung tissue lysate 4000ug.

Immunofluorescent analysis of HepG2 cells, using YWHAQ antibody 14503-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Immunofluorescent analysis of HepG2 cells, using YWHAQ antibody 14503-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

1X10^6 HeLa cells were stained with 0.2ug 14-3-3 antibody (14503-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.