14-3-3 Antibody
Rabbit Polyclonal
Catalog number: 14503-1-AP
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Applications
| Tested applications | ELISA, WB, IF, IP |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | human brain tissue, HeLa cells, Jurkat cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue, mouse lung tissue |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:500-1:5000 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP result of anti-YWHAQ (14-3-3; 14503-1-AP for IP and Detection) with mouse lung tissue lysate.
human brain tissue were subjected to SDS PAGE followed by western blot with 14503-1-AP(14-3-3 Antibody) at dilution of 1:1500
Immunofluorescent analysis of HepG2 cells, using YWHAQ antibody 14503-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | 14-3-3 fusion protein ag5959 | Full name | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide |
|
Calculated molecular weight |
28kd |
Observed molecular
weight |
31kd |
|
GenBank accession number |
BC056867 | Gene ID (NCBI) | 10971 |
| Gene symbol | YWHAQ | Synonyms | 14-3-3; 1C5; HS1 |
