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RCL1 Polyclonal antibody

RCL1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 15330-1-AP

Synonyms

RCL1, RNAC, RPC2, RPCL1, RTC2

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using RCL1 Polyclonal antibody (15330-1-AP)

    WB analysis of A431 using 15330-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RCL1 Polyclonal antibody (15330-1-AP)

    WB analysis of MCF-7 using 15330-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using RCL1 Polyclonal antibody (15330-1-AP)

    WB analysis of A375 using 15330-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RCL1 Polyclonal antibody (15330-1-AP)

    WB analysis of HepG2 using 15330-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using RCL1 Polyclonal antibody (15330-1-AP)

    WB analysis of A375 using 15330-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using RCL1 Polyclonal antibody (15330-1-AP)

    WB analysis of A431 using 15330-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using RCL1 Polyclonal antibody (15330-1-AP)

    IF Staining of A375 using 15330-1-AP

    Immunofluorescent analysis of A375 cells using 15330-1-AP (RCL1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA431 cells, HepG2 cells, A375 cells, MCF-7 cells
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15330-1-AP targets RCL1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RCL1 fusion protein Ag7058
Full Name RNA terminal phosphate cyclase-like 1
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC001025
Gene Symbol RCL1
Gene ID (NCBI) 10171
RRIDAB_2180010
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RCL1 antibody 15330-1-APDownload protocol
IF protocol for RCL1 antibody 15330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Mol Cell Biol

Stability and function of RCL1 are dependent on interaction with BMS1

Authors - Yong Wang
View Article
humanWB,IF

Cancer Cell Int

Rcl1 suppresses tumor progression of hepatocellular carcinoma: a comprehensive analysis of bioinformatics and in vitro experiments.

Authors - Yu Jiaze
View Article