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ABLIM2 Polyclonal antibody

ABLIM2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22433-1-AP

Synonyms

abLIM 2, ABLIM2, Actin binding LIM protein 2, KIAA1808

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ABLIM2 Polyclonal antibody (22433-1-AP)

    WB analysis of mouse brain using 22433-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22433-1-AP (ABLIM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using ABLIM2 Polyclonal antibody (22433-1-AP)

    WB analysis of mouse skeletal muscle using 22433-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22433-1-AP (ABLIM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ABLIM2 Polyclonal antibody (22433-1-AP)

    IP experiment of mouse brain using 22433-1-AP

    IP result of anti-ABLIM2 (IP:22433-1-AP, 4ug; Detection:22433-1-AP 1:1000) with mouse brain tissue lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ABLIM2 Polyclonal antibody (22433-1-AP)

    IHC staining of mouse skeletal muscle using 22433-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22433-1-AP (ABLIM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ABLIM2 Polyclonal antibody (22433-1-AP)

    IHC staining of mouse skeletal muscle using 22433-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22433-1-AP (ABLIM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using ABLIM2 Polyclonal antibody (22433-1-AP)

    IHC staining of human skeletal muscle using 22433-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22433-1-AP (ABLIM2 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using ABLIM2 Polyclonal antibody (22433-1-AP)

    IHC staining of human skeletal muscle using 22433-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22433-1-AP (ABLIM2 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ABLIM2 Polyclonal antibody (22433-1-AP)

    IHC staining of human kidney using 22433-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 22433-1-AP (ABLIM2 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ABLIM2 Polyclonal antibody (22433-1-AP)

    IHC staining of human kidney using 22433-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 22433-1-AP (ABLIM2 Antibody) at dilution of 1:50.

Tested Applications

Positive WB detected inmouse brain tissue, mouse skeletal muscle tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse skeletal muscle tissue, human skeletal muscle tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22433-1-AP targets ABLIM2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABLIM2 fusion protein Ag18198
Full Name actin binding LIM protein family, member 2
Calculated Molecular Weight 611 aa, 68 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC122567
Gene Symbol ABLIM2
Gene ID (NCBI) 84448
RRIDAB_11232422
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ABLIM2 antibody 22433-1-APDownload protocol
IHC protocol for ABLIM2 antibody 22433-1-APDownload protocol
IP protocol for ABLIM2 antibody 22433-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Joleen (Verified Customer) (06-10-2019)

Cell lysate on the left lane expressed GFP-ABLIM2 while on the right lane was wildtype cells. Antibody recognizes the GFP tagged construct ABLIM2 really well.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
ABLIM2 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:22433-1-AP)