Filter: AllWBIPIHCIF-PIF/ICCFC (Intra)

WB analysis using 23660-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 23660-1-AP (alpha Actin antibody) at dilution of 1:150000 incubated at room temperature for 1.5 hours.
IP experiment of mouse skeletal muscle using 23660-1-AP
IP result of anti-Alpha Actin (IP:23660-1-AP, 4ug; Detection:23660-1-AP 1:4000) with mouse skeletal muscle tissue lysate 2200ug.
IHC staining of human colon using 23660-1-AP
Immunohistochemical analysis of paraffin-embedded human colon slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.
IHC staining of human colon using 23660-1-AP
Immunohistochemical analysis of paraffin-embedded human colon slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.
IHC staining of human skeletal muscle using 23660-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.
IHC staining of human skeletal muscle using 23660-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.
IHC staining of human heart using 23660-1-AP
Immunohistochemical analysis of paraffin-embedded human heart slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.
IHC staining of human heart using 23660-1-AP
Immunohistochemical analysis of paraffin-embedded human heart slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.
IF Staining of mouse heart using 23660-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Alpha Actin antibody (23660-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse heart using 23660-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Alpha Actin antibody (23660-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse skeletal muscle using 23660-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Alpha Actin antibody (23660-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of H9C2 using 23660-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using Alpha Actin antibody (23660-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of C2C12 using 23660-1-AP
1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Alpha Actin (23660-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Alpha Actin Polyclonal antibody

Name: Alpha Actin Polyclonal antibody
Brand: Proteintech
SKU: 23660-1-AP
Rating: 4.00 (1 reviews)

Alpha Actin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 23660-1-AP

Publications(182)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Synonyms

ACTA1, α actin, ACTA, ACTA1 skeletal Muscle Actin, Actin alpha skeletal muscle

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (182)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected in	mouse skeletal muscle tissue
Positive IHC detected in	human colon tissue, human heart tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse heart tissue, mouse skeletal muscle tissue
Positive IF/ICC detected in	H9C2 cells
Positive FC (Intra) detected in	C2C12 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:20000-1:100000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:200
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 158 publications below
IHC	See 4 publications below
IF	See 20 publications below

Product Information

23660-1-AP targets Alpha Actin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag19151 Product name: Recombinant human ACTA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-377 aa of BC012597 Sequence: MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF Predict reactive species
Full Name	actin, alpha 1, skeletal muscle
Calculated Molecular Weight	42 kDa
Observed Molecular Weight	39-42 kDaa
GenBank Accession Number	BC012597
Gene Symbol	ACTA1
Gene ID (NCBI)	58
RRID	AB_2879307
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	P68133
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
FC protocol for Alpha Actin antibody 23660-1-AP	Download protocol
IF protocol for Alpha Actin antibody 23660-1-AP	Download protocol
IHC protocol for Alpha Actin antibody 23660-1-AP	Download protocol
IP protocol for Alpha Actin antibody 23660-1-AP	Download protocol
WB protocol for Alpha Actin antibody 23660-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Metab QDPR deficiency drives immune suppression in pancreatic cancer Authors - Ji Liu View Article
mouse,human	WB	Nat Commun Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma Authors - Weiwei Dai View Article
	WB	J Exp Med Tumor cell-intrinsic Piezo2 drives radioresistance by impairing CD8+ T cell stemness maintenance Authors - Naijun Miao View Article
mouse	WB	Acta Pharm Sin B A novel SIRT6 activator ameliorates neuroinflammation and ischemic brain injury via EZH2/FOXC1 axis. Authors - Tailin He View Article
		Redox Biol Endothelial H2S-AMPK dysfunction upregulates the angiocrine factor PAI-1 and contributes to lung fibrosis Authors - Xiangqi Chen View Article
mouse	WB	Transl Res Different roles of BAG3 in cardiac physiological hypertrophy and pathological remodeling. Authors - Pengyu Jia View Article