Home
Products
Primary Antibodies
Alpha smooth muscle actin Polyclonal antibody (14395-1-AP)

Featured Product
KD/KO Validated

Alpha smooth muscle actin Polyclonal antibody

Alpha smooth muscle actin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Cat No. 14395-1-AP

Publications (2320)

Reviews (5)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, ELISA, competitive binding assay, Western Blot

Synonyms

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, a SMA

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIPIHCIF-PIF-FroIF/ICCFC (Intra)

WB analysis using 14395-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14395-1-AP (smooth muscle actin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of A549 using 14395-1-AP
WB result of smooth muscle actin antibody (14395-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-smooth muscle actin transfected A549 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 14395-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14395-1-AP (smooth muscle actin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human placenta using 14395-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of mouse liver using 14395-1-AP
IP result of anti-smooth muscle actin (IP:14395-1-AP, 3ug; Detection:14395-1-AP 1:1000) with mouse liver tissue lysate 6000ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human appendicitis using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14395-1-AP (Alpha smooth muscle actin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody at dilution of 1:5000 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse colon using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14395-1-AP (Alpha smooth muscle actin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse colon using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14395-1-AP (Alpha smooth muscle actin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human appendicitis using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14395-1-AP (Alpha smooth muscle actin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody at dilution of 1:5000 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of stromal tumor using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of stromal tumor using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human hysteromyoma using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human hysteromyoma using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of human colon using 14395-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Alpha smooth muscle actin antibody (14395-1-AP) at dilution of 1:4000 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of human colon using 14395-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Alpha smooth muscle actin antibody (14395-1-AP) at dilution of 1:4000 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse heart using 14395-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Alpha smooth muscle actin antibody (14395-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse small intestine using 14395-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using smooth muscle actin antibody (14395-1-AP) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse colon using 14395-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using smooth muscle actin antibody (14395-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of C2C12 using 14395-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using smooth muscle actin antibody (14395-1-AP) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of H9C2 using 14395-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using smooth muscle actin antibody (14395-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of C2C12 using 14395-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using smooth muscle actin antibody (14395-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of C2C12 using 14395-1-AP
1x10^6 C2C12 cells were intracellularly stained with 0.4 ug smooth muscle actin Polyclonal antibody (14395-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for Alpha smooth muscle actin in ?

Overview

Tested Applications
Product Information
Publications (2320)
Protocols
Reviews (5)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse colon tissue, human placenta tissue, A549 cells, HUVEC cells, rat colon tissue, mouse heart tissue, rat heart tissue, mouse liver tissue, mouse kidney tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human appendicitis tissue, human colon tissue, human heart tissue, human hysteromyoma tissue, human liver cancer tissue, mouse colon tissue, stromal tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse heart tissue, mouse small intestine tissue, human colon tissue
Positive IF-Fro detected in	mouse colon tissue
Positive IF/ICC detected in	C2C12 cells, H9C2 cells
Positive FC (Intra) detected in	C2C12 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:4000-1:16000
Immunofluorescence (IF)-P	IF-P : 1:1000-1:4000
Immunofluorescence (IF)-FRO	IF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:800-1:3200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14395-1-AP targets Alpha smooth muscle actin in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, ELISA, competitive binding assay, Western Blot applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, rabbit, canine, monkey, chicken, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5886 Product name: Recombinant human ACTA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-377 aa of BC017554 Sequence: MCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF Predict reactive species
Full Name	actin, alpha 2, smooth muscle, aorta
Calculated Molecular Weight	377 aa, 42 kDa
Observed Molecular Weight	42 kDa
GenBank Accession Number	BC017554
Gene Symbol	Alpha smooth muscle actin
Gene ID (NCBI)	59
RRID	AB_2223009
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P62736
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.

Protocols

Product Specific Protocols
FC protocol for Alpha smooth muscle actin antibody 14395-1-AP	Download protocol
IF protocol for Alpha smooth muscle actin antibody 14395-1-AP	Download protocol
IHC protocol for Alpha smooth muscle actin antibody 14395-1-AP	Download protocol
IP protocol for Alpha smooth muscle actin antibody 14395-1-AP	Download protocol
WB protocol for Alpha smooth muscle actin antibody 14395-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

α-SMA (Alpha smooth muscle actin) polyclonal antibody (Cat# 14395-1-AP) has 2,320 citations published in journals including Nature Communications, Nature Genetics, Nature Cell Biology, Cell Metabolism, Cancer Cell, Molecular Cancer, Advanced Materials, Advanced Science, Biomaterials, ACS Nano, Theranostics, and Journal of Nanobiotechnology. Research fields encompass fibrosis (liver, renal, pulmonary, cardiac), oncology, wound healing, cardiovascular disease, tissue engineering, nanomedicine, and metabolism.

Species	Application	Title
mouse	IF	Signal Transduct Target Ther Porphyromonas gingivalis aggravates atherosclerotic plaque instability by promoting lipid-laden macrophage necroptosis Authors - Xiaofei Huang View Article
human	IF	Cancer Cell Pan-cancer human brain metastases atlas at single-cell resolution Authors - Xudong Xing View Article
		Cell Meningeal blood vessel blockage enhances anti-glioblastoma immunity. Authors - Yixin Gao View Article
mouse,human	IHC,IF	Mol Cancer CREB3L1 promotes tumor growth and metastasis of anaplastic thyroid carcinoma by remodeling the tumor microenvironment Authors - Zongfu Pan View Article
	WB	Mol Cancer Transcriptional landscape and predictive potential of long noncoding RNAs in peritoneal recurrence of gastric cancer Authors - Xiao-Xia Cai View Article
		Mol Cancer Modulation of tumor-derived exosomes and reprogramming of cancer-associated fibroblasts for colorectal cancer therapy. Authors - Susu Han View Article

Reviews

5.0 out of 5

Add a review

5 verified customer reviews

What customers say

All five reviewers gave 5-star ratings, praising the antibody's high specificity, sensitivity, and reliable, reproducible results. It was successfully used for immunohistochemistry and immunofluorescence across diverse samples including kidney, prostate, fibroblast cells, and zebrafish ocular muscles. Recommended dilutions ranged from 1:50 to 1:200. Reviewers noted strong signal quality and provided useful protocol details, such as Tris-EDTA pH 9.0 or NaCit pH 6 antigen retrieval for FFPE tissues. Overall, users consistently reported excellent performance with no negative feedback.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Alessandro (Verified Customer) (02-04-2025)	high specificity and sensitivity, providing reliable and reproducible results Applications: Immunohistochemistry, Immunofluorescence Primary Antibody Dilution: 1:200
James (Verified Customer) (09-22-2022)	Used by IF. Very well antibody. Applications: Immunohistochemistry Primary Antibody Dilution: 1 to 200 Cell Tissue Type: kidney Alpha smooth muscle actin Antibody Immunohistochemistry validation (1 to 200 dilution) in kidney (Cat no:14395-1-AP)
Wu (Verified Customer) (09-08-2022)	Used by IF. Really good signal Applications: Immunofluorescence Primary Antibody Dilution: 1:150 Cell Tissue Type: fibroblast cell Alpha smooth muscle actin Antibody Immunofluorescence validation (1:150 dilution) in fibroblast cell (Cat no:14395-1-AP)
Emma (Verified Customer) (11-29-2021)	Used by IF on FFPE prostate tissue. Tris-EDTA pH 9.0 antigen retrieval. Applications: Immunofluorescence Primary Antibody Dilution: 1:50 Cell Tissue Type: Prostate Alpha smooth muscle actin Antibody Immunofluorescence validation (1:50 dilution) in Prostate (Cat no:14395-1-AP)
Ryan (Verified Customer) (02-28-2019)	Tissue was fixed in PFA and antigen retrieval was done in NaCit ph=6. Applications: Immunohistochemistry, Immunofluorescence, Primary Antibody Dilution: 1:100 Cell Tissue Type: Zebrafish ocular muscles at 3dpf