ALDH1A1 Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IHC |
| Cited applications | ELISA, IF |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, mouse |
| Positive WB detected in | mouse liver tissue, HL-60 cells, human liver tissue, K-562 cells |
| Positive IHC detected in | human eye |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 |
IP = Immunoprecipitation
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15910-1-AP(ALDH1A1 Antibody) at dilution of 1:1500
Immunohistochemistry of paraffin-embedded human eye using 15910-1-AP(ALDH1A1 Antibody) at Dilution 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human eye using 15910-1-AP(ALDH1A1 Antibody) at Dilution 1:100 (under 40x lens)
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | ALDH1A1 fusion protein ag8665 | Full name | aldehyde dehydrogenase 1 family, member A1 |
|
Calculated molecular weight |
501aa,55kd |
Observed molecular
weight |
50-55kd |
|
GenBank accession number |
BC001505 | Gene ID (NCBI) | 216 |
| Gene symbol | ALDH1A1 | Synonyms | ALDC; ALDH-E1; ALDH1; ALDH11; MGC2318; PUMB1; RALDH1 |
ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001). This antibody is specific to ALDH1A1. |
