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  • Featured Product
  • KD/KO Validated

ANTXR1 Polyclonal antibody

ANTXR1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15091-1-AP

Synonyms

anthrax toxin receptor 1, ANTXR1, ATR, TEM8, Tumor endothelial marker 8

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using ANTXR1 Polyclonal antibody (15091-1-AP)

    WB analysis of mouse testis using 15091-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ANTXR1 Polyclonal antibody (15091-1-AP)

    WB analysis of HeLa using 15091-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-13 cells using ANTXR1 Polyclonal antibody (15091-1-AP)

    WB analysis of PC-13 using 15091-1-AP

    PC-13 cells were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using ANTXR1 Polyclonal antibody (15091-1-AP)

    WB analysis of mouse lung using 15091-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ANTXR1 Polyclonal antibody (15091-1-AP)

    IHC staining of human colon cancer using 15091-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15091-1-AP (TEM8 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ANTXR1 Polyclonal antibody (15091-1-AP)

    IHC staining of human colon cancer using 15091-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15091-1-AP (TEM8 antibody at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ANTXR1 Polyclonal antibody (15091-1-AP)

    IF Staining of HeLa using 15091-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15091-1-AP (TEM8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse testis tissue, HeLa cells, mouse lung tissue, PC-13 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15091-1-AP targets ANTXR1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANTXR1 fusion protein Ag4626
Full Name anthrax toxin receptor 1
Calculated Molecular Weight 63 kDa
Observed Molecular Weight 85 kDa, 80 kDa
GenBank Accession NumberBC012074
Gene Symbol ANTXR1
Gene ID (NCBI) 84168
RRIDAB_2056891
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tumor endothelial marker 8 (TEM8, ANTXR1) and capillary morphogenesis protein 2 (CMG2, ANTXR2) are the two well-characterized anthrax toxin receptors, each containing a von Willebrand factor A (vWA) domain responsible for anthrax protective antigen (PA) binding (PMID: 23271637). TEM8 is a highly-conserved single-pass cell-surface protein overexpressed on tumor-infiltrating vasculature, and is thought to play a positive role in endothelial cell activities related to angiogenesis (PMID: 11559528; 15777794 ). The long isoforms of TEM8 have been identified as an 80-85 kDa doublet, and experimental deglycosylation reduced the molecular mass to 70 kDa, indicating that TEM8 undergoes multiple glycosylations (PMID: 14871805; 15689409).

Protocols

Product Specific Protocols
WB protocol for ANTXR1 antibody 15091-1-APDownload protocol
IHC protocol for ANTXR1 antibody 15091-1-APDownload protocol
IF protocol for ANTXR1 antibody 15091-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Am J Transl Res

MicroRNA-26b-3p/ANTXR1 signaling modulates proliferation, migration, and apoptosis of glioma.

Authors - Fei Geng
View Article
humanWB

Cancer Manag Res

KLF7 Promotes Gastric Carcinogenesis Through Regulation of ANTXR1.

Authors - Yuanchun Li
  • KD Validated
View Article
humanWB

Comput Math Methods Med

miR-381-3p Involves in Glioma Progression by Suppressing Tumor-Promoter Factor ANTXR1.

Authors - Zhiqiang Dong
View Article