ANXA2 Antibody
Rabbit Polyclonal
Catalog number: 11256-1-AP
Ask a question about this product click here
Loading price...
Applications
| Tested applications | ELISA, WB, IHC, IF, IP |
| Cited applications | IHC, WB |
| Species specificity | human, mouse; other species not tested. |
| Cited species | chicken, human, mosue |
| Positive WB detected in | HeLa cells, K-562 cells, NIH/3T3 cells |
| Positive IHC detected in | human colon cancer |
| Positive IF detected in | Hela cells |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:50-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP result of ANXA2 antibody (11256-1-AP for IP and Detection) with HeLa cell lysate.
HeLa cells were subjected to SDS PAGE followed by western blot with 11256-1-AP(ANXA2 Antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human colon cancer using 11256-1-AP(ANXA2 Antibody) at Dilution 1:100 (under 10x lens)
Immunofluorescent analysis of Hela cells, using ANXA2 antibody 11256-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | ANXA2 fusion protein ag1777 | Full name | annexin A2 |
|
Calculated molecular weight |
339aa,39kd |
Observed molecular
weight |
39 kDa |
|
GenBank accession number |
BC016774 | Gene ID (NCBI) | 302 |
| Gene symbol | ANXA2 | Synonyms | ANX2; ANX2L4; CAL1H; LIP2; LPC2; LPC2D; P36; PAP-IV |
Background
Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2. |
