ANXA2 AntibodyRabbit Polyclonal
|Tested applications||ELISA, WB, IHC, IF, IP|
|Cited applications||IHC, WB|
|Species specificity||human, mouse; other species not tested.|
|Cited species||chicken, human, mosue|
|Positive WB detected in||HeLa cells, K-562 cells, NIH/3T3 cells|
|Positive IHC detected in||human colon cancer|
|Positive IF detected in||Hela cells|
|Recommended dilution||WB: 1:200-1:2000|
IP = Immunoprecipitation
- IP result of ANXA2 antibody (11256-1-AP for IP and Detection) with HeLa cell lysate.
- HeLa cells were subjected to SDS PAGE followed by western blot with 11256-1-AP(ANXA2 Antibody) at dilution of 1:500
- Immunohistochemistry of paraffin-embedded human colon cancer using 11256-1-AP(ANXA2 Antibody) at Dilution 1:100 (under 10x lens)
- Immunofluorescent analysis of Hela cells, using ANXA2 antibody 11256-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||ANXA2 fusion protein ag1777||Full name||annexin A2|
|BC016774||Gene ID (NCBI)||302|
|Gene symbol||ANXA2||Synonyms||ANX2; ANX2L4; CAL1H; LIP2; LPC2; LPC2D; P36; PAP-IV|
Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.