APPL1 Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IHC |
| Cited applications | IHC, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive WB detected in | human brain tissue, mouse ovary tissue |
| Positive IHC detected in | human ovary tumor |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 |
IP = Immunoprecipitation
human brain tissue were subjected to SDS PAGE followed by western blot with 12639-1-AP(APPL1 Antibody) at dilution of 1:1500
Immunohistochemistry of paraffin-embedded human ovary tumor using 12639-1-AP(APPL1 Antibody) at Dilution 1:50 (under 10x lens)
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | APPL1 fusion protein ag3334 | Full name | adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 |
|
Calculated molecular weight |
709aa,80kd |
Observed molecular
weight |
80kd |
|
GenBank accession number |
BC028599 | Gene ID (NCBI) | 26060 |
| Gene symbol | APPL1 | Synonyms | APPL; DIP13alpha |
Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of insulin signaling, plays a key role in the regulation of insulin secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the insulin-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092]. |
