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ARPC5L Polyclonal antibody

ARPC5L Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 22025-1-AP

Synonyms

ARC16 2, ARPC5L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARPC5L Polyclonal antibody (22025-1-AP)

    WB analysis using 22025-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using ARPC5L Polyclonal antibody (22025-1-AP)

    WB analysis of human brain using 22025-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using ARPC5L Polyclonal antibody (22025-1-AP)

    WB analysis of NIH/3T3 using 22025-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using ARPC5L Polyclonal antibody (22025-1-AP)

    WB analysis of mouse lung using 22025-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ARPC5L Polyclonal antibody (22025-1-AP)

    IP experiment of mouse brain using 22025-1-AP

    IP result of anti-ARPC5L (IP:22025-1-AP, 4ug; Detection:22025-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ARPC5L Polyclonal antibody (22025-1-AP)

    IHC staining of human placenta using 22025-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ARPC5L Polyclonal antibody (22025-1-AP)

    IHC staining of human placenta using 22025-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using ARPC5L Polyclonal antibody (22025-1-AP)

    IHC staining of human cerebellum using 22025-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using ARPC5L Polyclonal antibody (22025-1-AP)

    IHC staining of human cerebellum using 22025-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ARPC5L Polyclonal antibody (22025-1-AP)

    IF Staining of MCF-7 using 22025-1-AP

    Immunofluorescent analysis of MCF-7 cells using 22025-1-AP (ARPC5L antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • Flow cytometry (FC) experiment of MCF-7 cells using ARPC5L Polyclonal antibody (22025-1-AP)

    FC experiment of MCF-7 using 22025-1-AP

    1X10^6 MCF-7 cells were stained with 0.2ug ARPC5L antibody (22025-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, NIH/3T3 cells, mouse lung tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman placenta tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22025-1-AP is ARPC5L Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARPC5L fusion protein Ag16881
Full Name actin related protein 2/3 complex, subunit 5-like
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 17-20 kDa
GenBank Accession NumberBC000798
Gene Symbol ARPC5L
Gene ID (NCBI) 81873
RRIDAB_11182273
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARPC5L, also known as ARC16-2, belongs to the ARPC5 family. ARPC5L is involved in Arp2/3 complex-mediated actin nucleation and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. ARPC5L-containing Arp2/3 complexes are required for the peripheral positioning of nuclei in myofibers (PMID: 28892082). The molecular mass of ARPC5L is 17-20 kDa.

Protocols

Product Specific Protocols
WB protocol for ARPC5L antibody 22025-1-APDownload protocol
IHC protocol for ARPC5L antibody 22025-1-APDownload protocol
IF protocol for ARPC5L antibody 22025-1-APDownload protocol
IP protocol for ARPC5L antibody 22025-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Pigment Cell Melanoma Res

YAP facilitates melanoma migration through regulation of actin-related protein 2/3 complex subunit 5 (ARPC5)

Authors - Jason W. Lui
View Article