BCL2 Antibody
Rabbit Polyclonal
Catalog number: 12789-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IP |
| Cited applications | WB |
| Species specificity | human; other species not tested. |
| Cited species | human, mouse, rabbit |
| Positive WB detected in | HL-60 cells, HepG2 cells, Jurkat cells, U-937 cells |
| Positive IHC detected in | human breast cancer |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP result of anti-BCL2 (12789-1-AP for IP and Detection) with HepG2 cell lysate.
HL-60 cells were subjected to SDS PAGE followed by western blot with 12789-1-AP(BCL2 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human breast cancer using 12789-1-AP(BCL2 Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer using 12789-1-AP(BCL2 Antibody) at Dilution 1:50 (under 40x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | BCL2 fusion protein ag3508 | Full name | B-cell CLL/lymphoma 2 |
|
Calculated molecular weight |
26kd |
Observed molecular
weight |
25-30kd |
|
GenBank accession number |
BC027258 | Gene ID (NCBI) | 596 |
| Gene symbol | BCL2 | Synonyms | Bcl-2 |
Background
BCL2 belongs to the Bcl-2 family. It is a suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability (PMID: 10365962). It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PMID: 10444588). |
