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BEND3 Polyclonal antibody

BEND3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23101-1-AP

Synonyms

BEN domain containing 3, BEND3, KIAA1553, RP11 59I9.2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BEND3 Polyclonal antibody (23101-1-AP)

    WB analysis using 23101-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23101-1-AP (BEND3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BEND3 Polyclonal antibody (23101-1-AP)

    IHC staining of human liver cancer using 23101-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23101-1-AP (BEND3 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BEND3 Polyclonal antibody (23101-1-AP)

    IHC staining of human liver cancer using 23101-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23101-1-AP (BEND3 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using BEND3 Polyclonal antibody (23101-1-AP)

    IF Staining of HEK-293 using 23101-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using BEND3 antibody (23101-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23101-1-AP targets BEND3 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BEND3 fusion protein Ag19137
Full Name BEN domain containing 3
Calculated Molecular Weight 828 aa, 94 kDa
Observed Molecular Weight94 kDa
GenBank Accession NumberBC131628
Gene Symbol BEND3
Gene ID (NCBI) 57673
RRIDAB_2879210
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BEND3 is a quadruple BEN domain-containing protein that associates with heterochromatin and functions as a transcriptional repressor. BEND3 is highly expressed in pluripotent cells, and the induction of differentiation results in the down-regulation of BEND3. (PMID: 35217604, PMID: 25600804)

Protocols

Product Specific Protocols
WB protocol for BEND3 antibody 23101-1-APDownload protocol
IHC protocol for BEND3 antibody 23101-1-APDownload protocol
IF protocol for BEND3 antibody 23101-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols