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  • Featured Product
  • KD/KO Validated

C6orf211/ARMT1 Polyclonal antibody

C6orf211/ARMT1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25124-1-AP

Synonyms

Acidic residue methyltransferase 1, ARMT1, C6orf211, Protein-glutamate O-methyltransferase

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using C6orf211/ARMT1 Polyclonal antibody (25124-1-AP)

    WB analysis of MCF-7 using 25124-1-AP

    WB result of C6orf211/ARMT1 antibody (25124-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C6orf211/ARMT1 transfected MCF-7 cells.

  • Western Blot (WB) analysis of MCF-7 cells using C6orf211/ARMT1 Polyclonal antibody (25124-1-AP)

    WB analysis of MCF-7 using 25124-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25124-1-AP (C6orf211 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using C6orf211/ARMT1 Polyclonal antibody (25124-1-AP)

    WB analysis of A431 using 25124-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 25124-1-AP (C6orf211 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of T-47D cells using C6orf211/ARMT1 Polyclonal antibody (25124-1-AP)

    WB analysis of T-47D using 25124-1-AP

    T-47D cells were subjected to SDS PAGE followed by western blot with 25124-1-AP (C6orf211 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using C6orf211/ARMT1 Polyclonal antibody (25124-1-AP)

    WB analysis of HEK-293 using 25124-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25124-1-AP (C6orf211 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using C6orf211/ARMT1 Polyclonal antibody (25124-1-AP)

    IHC staining of human testis using 25124-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25124-1-AP (C6orf211 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using C6orf211/ARMT1 Polyclonal antibody (25124-1-AP)

    IHC staining of human testis using 25124-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25124-1-AP (C6orf211 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C6orf211/ARMT1 Polyclonal antibody (25124-1-AP)

    IHC staining of human breast cancer using 25124-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25124-1-AP (C6orf211 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C6orf211/ARMT1 Polyclonal antibody (25124-1-AP)

    IHC staining of human breast cancer using 25124-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25124-1-AP (C6orf211 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using C6orf211/ARMT1 Polyclonal antibody (25124-1-AP)

    IF Staining of MCF-7 using 25124-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 25124-1-AP (C6orf211 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells, A431 cells, T-47D cells
Positive IHC detected inhuman testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25124-1-AP targets C6orf211/ARMT1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C6orf211/ARMT1 fusion protein Ag19090
Full Name chromosome 6 open reading frame 211
Calculated Molecular Weight 441 aa, 51 kDa
Observed Molecular Weight 50-51 kDa
GenBank Accession NumberBC011348
Gene Symbol C6orf211
Gene ID (NCBI) 79624
RRIDAB_2879911
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C6orf211/ARMT1 antibody 25124-1-APDownload protocol
IHC protocol for C6orf211/ARMT1 antibody 25124-1-APDownload protocol
IF protocol for C6orf211/ARMT1 antibody 25124-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols