calreticulin Antibody
Rabbit Polyclonal
Catalog number: 10292-1-AP
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Applications
| Tested applications | ELISA, WB, IHC |
| Cited applications | IF, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, rat |
| Positive WB detected in | COLO 320 cells, HeLa cells, human skeletal muscle tissue, MCF7 cells |
| Positive IHC detected in | human ovary tumor |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:20-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
COLO 320 cells were subjected to SDS PAGE followed by western blot with 10292-1-AP(calreticulin Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human ovary tumor using 10292-1-AP(calreticulin Antibody) at Dilution 1:50 (under 10x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | calreticulin fusion protein ag0325 | Full name | calreticulin |
|
Calculated molecular weight |
60kd |
Observed molecular
weight |
55-60kd |
|
GenBank accession number |
BC002500 | Gene ID (NCBI) | 811 |
| Gene symbol | CALR | Synonyms | CRT; FLJ26680; RO; SSA; cC1qR |
Background
CALR,also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is a ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 60 kD by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment. |
