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CARD9 Polyclonal antibody

CARD9 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 10669-1-AP

Synonyms

CARD9, hCARD9



Tested Applications

Positive WB detected inHL-60 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10669-1-AP targets CARD9 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CARD9 fusion protein Ag1057
Full Name caspase recruitment domain family, member 9
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 50-59 kDa
GenBank Accession NumberBC008877
Gene Symbol CARD9
Gene ID (NCBI) 64170
RRIDAB_2228323
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CARD9, a member of the caspase recruitment domain (CARD) protein family, plays an important regulatory role in cell apoptosis. Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CARD9 is highly expressed in spleen and regulate activation of NF-kappa-B by interaction with postive regulator of apoptosis BCL10 via the CARD domain.

Protocols

Product Specific Protocols
WB protocol for CARD9 antibody 10669-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Blood

Invasive fungal infection and impaired neutrophil killing in human CARD9 deficiency.

Authors - Drewniak Agata A
humanWB

PLoS Pathog

CARD9-Dependent Neutrophil Recruitment Protects against Fungal Invasion of the Central Nervous System.

Authors - Rebecca A Drummond
humanWB

J Clin Immunol

Inherited CARD9 Deficiency in a Child with Invasive Disease Due to Exophiala dermatitidis and Two Older but Asymptomatic Siblings.

Authors - Yusuke Imanaka
mouseWB

Transl Stroke Res

Inhibition of Dectin-1 Ameliorates Neuroinflammation by Regulating Microglia/Macrophage Phenotype After Intracerebral Hemorrhage in Mice.

Authors - Xiongjie Fu
humanWB

Plast Reconstr Surg

Chronic Candida albicans Meningitis in a 4-Year-Old Girl with a Homozygous Mutation in the CARD9 Gene (Q295X).

Authors - Martin Herbst
mouseWB

Front Immunol

TREM1 Regulates Neuroinflammatory Injury by Modulate Proinflammatory Subtype Transition of Microglia and Formation of Neutrophil Extracellular Traps via Interaction With SYK in Experimental Subarachnoid Hemorrhage.

Authors - Xinyan Wu