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  • Featured Product
  • KD/KO Validated

CCM2 Polyclonal antibody

CCM2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 26270-1-AP

Synonyms

C7orf22, CCM2, Malcavernin, OSM, PP10187

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using CCM2 Polyclonal antibody (26270-1-AP)

    WB analysis of MCF-7 using 26270-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26270-1-AP (CCM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CCM2 Polyclonal antibody (26270-1-AP)

    IHC staining of human liver cancer using 26270-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26270-1-AP (CCM2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CCM2 Polyclonal antibody (26270-1-AP)

    IHC staining of human liver cancer using 26270-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26270-1-AP (CCM2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CCM2 Polyclonal antibody (26270-1-AP)

    IF Staining of MCF-7 using 26270-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 26270-1-AP (CCM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26270-1-AP is CCM2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCM2 fusion protein Ag24233
Full Name cerebral cavernous malformation 2
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC004903
Gene Symbol CCM2
Gene ID (NCBI) 83605
RRIDAB_2880454
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CCM2 antibody 26270-1-APDownload protocol
IHC protocol for CCM2 antibody 26270-1-APDownload protocol
IF protocol for CCM2 antibody 26270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem J

CCM2 and PAK4 act downstream of atrial natriuretic peptide signaling to promote cell spreading.

Authors - Koichi Miura
  • KD Validated
View Article