Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 10571-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 10571-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 10571-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 10571-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 10571-1-AP
IP result of anti-CCT3 (IP:10571-1-AP, 4ug; Detection:10571-1-AP 1:15000) with mouse brain tissue lysate 1720 ug.
IHC staining of mouse liver using 10571-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10571-1-AP (CCT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 10571-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CCT3 antibody (10571-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of HepG2 using 10571-1-AP
Immunofluorescent analysis of HepG2 cells, using CCT3 antibody 10571-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
FC experiment of HepG2 using 10571-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CCT3 Polyclonal antibody (10571-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

CCT3 Polyclonal antibody

Name: CCT3 Polyclonal antibody
Brand: Proteintech
SKU: 10571-1-AP

CCT3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10571-1-AP

Publications(18)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

TCP 1 gamma, CCT gamma, CCTG, CCTgamma, CCT-gamma

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Publications (18)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, rat kidney tissue, HeLa cells, Jurkat cells, mouse brain tissue, mouse kidney tissue, U2OS cells, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 15 publications below
IHC	See 1 publications below
IF	See 4 publications below
IP	See 1 publications below
CoIP	See 2 publications below

Product Information

10571-1-AP targets CCT3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0843 Product name: Recombinant human CCT3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 246-545 aa of BC006501 Sequence: EYKKGESQTDIEITREEDFTRILQMEEEYIQQLCEDIIQLKPDVVITEKGISDLAQHYLMRANITAIRRVRKTDNNRIARACGARIVSRPEELREDDVGTGAGLLEIKKIGDEYFTFITDCKDPKACTILLRGASKEILSEVERNLQDAMQVCRNVLLDPQLVPGGGASEMAVAHALTEKSKAMTGVEQWPYRAVAQALEVIPRTLIQNCGASTIRLLTSLRAKHTQENCETWGVNGETGTLVDMKELGIWEPLAVKLQTYKTAVETAVLLLRIDDIVSGHKKKGDDQSRQGGAPDAGQE Predict reactive species
Full Name	chaperonin containing TCP1, subunit 3 (gamma)
Calculated Molecular Weight	60 kDa
Observed Molecular Weight	60 kDa
GenBank Accession Number	BC006501
Gene Symbol	CCT3
Gene ID (NCBI)	7203
RRID	AB_2073658
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P49368
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCT3 protein (full name: chaperonin containing TCP1 subunit 3, also known as the gamma subunit of TCP-1 protein) is an important member of the TCP-1 chaperonin family. As one of the eight essential subunits of the CCT/TRiC complex, its core function is to act as a molecular chaperone that assists in the correct folding of other cellular proteins under ATP hydrolysis. It is particularly involved in the folding process of key cytoskeletal components such as actin and tubulin, while also participating in important biological activities including ciliogenesis and telomere maintenance.

Protocols

Product Specific Protocols
FC protocol for CCT3 antibody 10571-1-AP	Download protocol
IF protocol for CCT3 antibody 10571-1-AP	Download protocol
IHC protocol for CCT3 antibody 10571-1-AP	Download protocol
IP protocol for CCT3 antibody 10571-1-AP	Download protocol
WB protocol for CCT3 antibody 10571-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nat Genet DYX1C1 is required for axonemal dynein assembly and ciliary motility. Authors - Tarkar Aarti A View Article
mouse	WB	Cell Kinetic Analysis of Protein Stability Reveals Age-Dependent Degradation. Authors - Erik McShane View Article
	WB,IF	Adv Sci (Weinh) Primate-Specific DAZ Regulates Translation of Cell Proliferation-Related mRNAs and is Essential for Maintenance of Spermatogonia Authors - Ningjing Ou View Article
mouse,human	WB	Proc Natl Acad Sci U S A BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly. Authors - Seo Seongjin S KD Validated View Article
human	WB	J Cell Biol CEP55 promotes cilia disassembly through stabilizing Aurora A kinase. Authors - Yu-Cheng Zhang View Article
human	WB,IF	Dev Biol Involvement of multimeric protein complexes in mediating the capacitation-dependent binding of human spermatozoa to homologous zonae pellucidae. Authors - Redgrove Kate A KA View Article