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Cirhin Polyclonal antibody

Cirhin Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11057-1-AP

Synonyms

CIRH1A, Cirhin, KIAA1988, NAIC, TEX292, UTP4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cirhin Polyclonal antibody (11057-1-AP)

    WB analysis using 11057-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 11057-1-AP (Cirhin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using Cirhin Polyclonal antibody (11057-1-AP)

    IP experiment of HepG2 using 11057-1-AP

    IP result of anti-Cirhin (IP:11057-1-AP, 4ug; Detection:11057-1-AP 1:700) with HepG2 cells lysate 2600 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Cirhin Polyclonal antibody (11057-1-AP)

    IHC staining of mouse brain using 11057-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11057-1-AP (Cirhin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Cirhin Polyclonal antibody (11057-1-AP)

    IHC staining of mouse brain using 11057-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11057-1-AP (Cirhin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inNeuro-2a cells
Positive IP detected inHepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11057-1-AP targets Cirhin in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cirhin fusion protein Ag1494
Full Name cirrhosis, autosomal recessive 1A (cirhin)
Calculated Molecular Weight 77 kDa
Observed Molecular Weight 70-77 kDa
GenBank Accession NumberBC009348
Gene Symbol CIRH1A
Gene ID (NCBI) 84916
RRIDAB_2877743
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cirhin is a protein that in humans is encoded by the CIRH1A gene. Mutation of this gene has been associated with North American Indian childhood cirrhosis, a severe intrahepatic cholestasis that results in transient neonatal jaundice, and progresses to periportal fibrosis and cirrhosis in childhood and adolescence (PMID: 12417987). Catalog#11057-1-AP is a rabbit polyclonal antibody raised against the full-length of human CIRHIN.

Protocols

Product Specific Protocols
WB protocol for Cirhin antibody 11057-1-APDownload protocol
IHC protocol for Cirhin antibody 11057-1-APDownload protocol
IP protocol for Cirhin antibody 11057-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols