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CMC1 Polyclonal antibody

CMC1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24030-1-AP

Synonyms

C3orf68, CMC1, Cmc1p

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CMC1 Polyclonal antibody (24030-1-AP)

    WB analysis using 24030-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24030-1-AP (CMC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using CMC1 Polyclonal antibody (24030-1-AP)

    IP experiment of K-562 using 24030-1-AP

    IP result of anti-CMC1 (IP:24030-1-AP, 4ug; Detection:24030-1-AP 1:300) with K-562 cells lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CMC1 Polyclonal antibody (24030-1-AP)

    IHC staining of human liver cancer using 24030-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24030-1-AP (CMC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CMC1 Polyclonal antibody (24030-1-AP)

    IHC staining of human liver cancer using 24030-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24030-1-AP (CMC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inK-562 cells, mouse liver tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 24030-1-AP is CMC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CMC1 fusion protein Ag21224
Full Name COX assembly mitochondrial protein homolog (S. cerevisiae)
Calculated Molecular Weight 106 aa, 12 kDa
Observed Molecular Weight 12-15 kDa
GenBank Accession NumberBC052644
Gene Symbol CMC1
Gene ID (NCBI) 152100
RRIDAB_2879409
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CMC1 antibody 24030-1-APDownload protocol
IHC protocol for CMC1 antibody 24030-1-APDownload protocol
IP protocol for CMC1 antibody 24030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

A novel defined cuproptosis-related gene signature for predicting the prognosis of colon adenocarcinoma

Authors - Bixian Luo
View Article