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CREB5 Polyclonal antibody

CREB5 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14196-1-AP

Synonyms

CRE BPA, CREB 5, CREB5, CREBPA

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HepG2 cells using CREB5 Polyclonal antibody (14196-1-AP)

    WB analysis of HepG2 using 14196-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14196-1-AP (CREB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CREB5 Polyclonal antibody (14196-1-AP)

    WB analysis of HeLa using 14196-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14196-1-AP (CREB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using CREB5 Polyclonal antibody (14196-1-AP)

    WB analysis of MCF-7 using 14196-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14196-1-AP (CREB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using CREB5 Polyclonal antibody (14196-1-AP)

    WB analysis of human brain using 14196-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14196-1-AP (CREB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, human brain tissue, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

14196-1-AP targets CREB5 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CREB5 fusion protein Ag5399
Full Name cAMP responsive element binding protein 5
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC059400
Gene Symbol CREB5
Gene ID (NCBI) 9586
RRIDAB_2083832
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CREB5 antibody 14196-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Hyperthermia

Hyperthermia promotes exosome secretion by regulating Rab7b while increasing drug sensitivity in adriamycin-resistant breast cancer.

Authors - Di Xu
View Article
humanWB

J Ovarian Res

Role of BMI1 in epithelial ovarian cancer: investigated via the CRISPR/Cas9 system and RNA sequencing.

Authors - Qianying Zhao
View Article
humanWB

PLoS One

AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing's sarcoma cells

Authors - Takao Kitagawa
View Article
humanWB

Cancer Med

Long noncoding RNA SNHG4 promotes the malignant progression of hepatocellular carcinoma through the miR-211-5p/CREB5 axis

Authors - Jiannan Qiu
View Article