CREM Polyclonal antibody

CREM Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12131-1-AP

Synonyms

CREM, hCREM 2, ICER, Inducible cAMP early repressor



Tested Applications

Positive WB detected inrat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12131-1-AP targets CREM in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CREM fusion protein Ag2775
Full Name cAMP responsive element modulator
Calculated Molecular Weight 332 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC017117
Gene Symbol CREM
Gene ID (NCBI) 1390
RRIDAB_2084260
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CREM antibody 12131-1-APDownload protocol
IHC protocol for CREM antibody 12131-1-APDownload protocol
IP protocol for CREM antibody 12131-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A druggable copper-signalling pathway that drives inflammation

Authors - Stéphanie Solier
mouseIF

Biofabrication

Testis on a chip - a microfluidic 3-dimensional culture system for the development of spermatogenesisin-vitro.

Authors - Ali AbuMadighem
mouseIF

Int J Mol Sci

Effect of Chemotherapy Cytarabine and Acute Myeloid Leukemia on the Development of Spermatogenesis at the Adult Age of Immature Treated Mice.

Authors - Bara'ah Khaleel
mouseIF

Int J Mol Sci

The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells.

Authors - Noy Bagdadi
mouseIHC,WB

Toxicol Appl Pharmacol

Reproductive effects of pubertal exposure to neonicotinoid thiacloprid in immature male mice

Authors - Yong Zou