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  • KD/KO Validated

CRIPT Polyclonal antibody

CRIPT Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11211-1-AP

Synonyms

CRIPT, HSPC139



Tested Applications

Positive WB detected inL02 cells
Positive IP detected inL02 cells
Positive IF detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11211-1-AP targets CRIPT in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRIPT fusion protein Ag1708
Full Name cysteine-rich PDZ-binding protein
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC018653
Gene Symbol CRIPT
Gene ID (NCBI) 9419
RRIDAB_2084731
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The clustering and immobilization of receptors and ion channels located at specific subcellular sites are believed to be mediated by intracellular proteins that attach these membrane proteins to the cytoskeleton. Receptors and ion channels interact with cytoplasmic proteins, which involved in the modulation or downstream signaling mechanisms of the receptor/ion channel. CRIPT is the protein that binds to the third PDZ (PDZ3) domain of PSD95, which binds to and clusters a variety of membrane proteins, including Shaker-type potassium channel subunits and NMDA receptor NR2 subunits, via its first 2 N-terminal PDZ domains

Protocols

Product Specific Protocols
WB protocol for CRIPT antibody 11211-1-APDownload protocol
IF protocol for CRIPT antibody 11211-1-APDownload protocol
IP protocol for CRIPT antibody 11211-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,CoIP

Mol Neurobiol

Interaction Between CRIPT and PSD-95 Is Required for Proper Dendritic Arborization in Hippocampal Neurons.

Authors - Anton Omelchenko
  • KD Validated
ratWB,IP

eNeuro

SAP97 Binding Partner CRIPT Promotes Dendrite Growth In Vitro and In Vivo.

Authors - Lei Zhang