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CTDSPL2 Polyclonal antibody

CTDSPL2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17532-1-AP

Synonyms

CTDSP like 2, CTDSPL2, HSPC058, HSPC129

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using CTDSPL2 Polyclonal antibody (17532-1-AP)

    WB analysis of MCF-7 using 17532-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using CTDSPL2 Polyclonal antibody (17532-1-AP)

    WB analysis of mouse testis using 17532-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse eye tissue using CTDSPL2 Polyclonal antibody (17532-1-AP)

    WB analysis of mouse eye using 17532-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using CTDSPL2 Polyclonal antibody (17532-1-AP)

    WB analysis of human brain using 17532-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CTDSPL2 Polyclonal antibody (17532-1-AP)

    WB analysis of HeLa using 17532-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CTDSPL2 Polyclonal antibody (17532-1-AP)

    WB analysis of HepG2 using 17532-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using CTDSPL2 Polyclonal antibody (17532-1-AP)

    WB analysis of Y79 using 17532-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CTDSPL2 Polyclonal antibody (17532-1-AP)

    WB analysis of HeLa using 17532-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using CTDSPL2 Polyclonal antibody (17532-1-AP)

    IP experiment of mouse testis using 17532-1-AP

    IP result of anti-CTDSPL2 (IP:17532-1-AP, 4ug; Detection:17532-1-AP 1:500) with mouse testis tissue lysate 4400ug.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using CTDSPL2 Polyclonal antibody (17532-1-AP)

    IHC staining of human endometrial cancer using 17532-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 17532-1-AP (CTDSPL2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CTDSPL2 Polyclonal antibody (17532-1-AP)

    IHC staining of human testis using 17532-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17532-1-AP (CTDSPL2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using CTDSPL2 Polyclonal antibody (17532-1-AP)

    IHC staining of human endometrial cancer using 17532-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 17532-1-AP (CTDSPL2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CTDSPL2 Polyclonal antibody (17532-1-AP)

    IHC staining of human testis using 17532-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17532-1-AP (CTDSPL2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CTDSPL2 Polyclonal antibody (17532-1-AP)

    IF Staining of HeLa using 17532-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 17532-1-AP (CTDSPL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF7 cells, HeLa cells, HepG2 cells, human brain tissue, mouse eye tissue, mouse testis tissue, Y79 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman endometrial cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

17532-1-AP targets CTDSPL2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTDSPL2 fusion protein Ag11581
Full Name CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase like 2
Calculated Molecular Weight 466 aa, 53 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC035744
Gene Symbol CTDSPL2
Gene ID (NCBI) 51496
RRIDAB_2087204
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTD small phosphatase-like protein 2 (CTDSPL2) is also named as HSPC058 and HSPC129, belongs to the CTDSPL2 family. It is a putative RNA-polymerase II carboxy-terminal domain (CTD) phosphatase, whose specific CTD phosphatase activity was verified in vitro, and may regulate the dynamic phosphorylation of RNAP II CTD.( PMID: 17487459) It can obviously improve the expression of ε- and γ-globin genes in K562 cells and CD34+ cells derived from UCB.( PMID: 20932329) This antibody is a rabbit polyclonal antibody raised against a region of human CTDSPL2.

Protocols

Product Specific Protocols
WB protocol for CTDSPL2 antibody 17532-1-APDownload protocol
IHC protocol for CTDSPL2 antibody 17532-1-APDownload protocol
IF protocol for CTDSPL2 antibody 17532-1-APDownload protocol
IP protocol for CTDSPL2 antibody 17532-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

BMC Cell Biol

CTD small phosphatase like 2 (CTDSPL2) can increase ε- and γ-globin gene expression in K562 cells and CD34+ cells derived from umbilical cord blood.

Authors - Ma Yan-Ni YN
View Article
humanWB

Cell Death Dis

MicroRNA-26a/b have protective roles in oral lichen planus.

Authors - Jie Du
View Article