CTSB Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IHC |
| Cited applications | |
| Species specificity | human, mouse; other species not tested. |
| Cited species | |
| Positive WB detected in | A375 cells, human brain tissue, human liver tissue, mouse brain tissue, NIH/3T3 cells, RAW 264.7 cells, HT-1080 cells |
| Positive IHC detected in | human breast cancer |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:20-1:200 |
IP = Immunoprecipitation
A375 cells were subjected to SDS PAGE followed by western blot with 12216-1-AP(CTSB Antibody) at dilution of 1:800
Immunohistochemistry of paraffin-embedded human breast cancer using 12216-1-AP(CTSB Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer using 12216-1-AP(CTSB Antibody) at Dilution 1:50 (under 40x lens)
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | CTSB fusion protein ag2898 | Full name | cathepsin B |
|
Calculated molecular weight |
229aa,38kd |
Observed molecular
weight |
38 kDa, 44 kDa |
|
GenBank accession number |
BC010240 | Gene ID (NCBI) | 1508 |
| Gene symbol | CTSB | Synonyms | APPS; CPSB |
CTSB(Cathepsin B) is also named as CPSB and belongs to the peptidase C1 family. It participates in intracellular degradation and turnover of proteins. Cathepsin B precursors found in human malignant ascites fluid do not possess mannose-rich carbohydrates suggesting that a defect in the post translational processing of carbohydrate moieties on tumor cathepsin B may be responsible for the release of cathepsin B observed in many tumor systems. Cathepsin B exists as both glycosylated and unglycosylated forms(PMID:1637335). In rat macrophages and hepatocytes pro- cathepsin B is 39 kDa (unglycosylated =35 kDa), whereas in human fibroblasts procathepsin B is 44.5-46kDa (unglycosylated=39kDa)(PMID:2097084). It can be detected the 43 kDa form of pro-CTSB, a single-chain intermediate of 31 kDa and the double-chain mature species of 25 kDa in mouse brain by western blot(PMID:20616152). This antibody is specific to CTSB. |
