Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis of NIH/3T3 using 66364-1-Ig
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66364-1-Ig (D2HGDH Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 66364-1-Ig
C6 cells were subjected to SDS PAGE followed by western blot with 66364-1-Ig (D2HGDH Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 66364-1-Ig
rat heart tissue were subjected to SDS PAGE followed by western blot with 66364-1-Ig (D2HGDH Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 66364-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66364-1-Ig (D2HGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 66364-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66364-1-Ig (D2HGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 66364-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using D2HGDH antibody (66364-1-Ig, Clone: 1E9B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of HepG2 using 66364-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using D2HGDH antibody (66364-1-Ig, Clone: 1E9B3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).
FC experiment of HepG2 using 66364-1-Ig
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human D2HGDH (66364-1-Ig, Clone:1E9B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

D2HGDH Monoclonal antibody

Name: D2HGDH Monoclonal antibody
Brand: Proteintech
SKU: 66364-1-Ig

D2HGDH Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 66364-1-Ig

Clone No.1E9B3

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

1E9B3, D2HGD, D-2-hydroxyglutarate dehydrogenase, mitochondrial, EC:1.1.99.39

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	NIH/3T3 cells, C6 cells, rat heart tissue
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66364-1-Ig targets D2HGDH in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag5036 Product name: Recombinant human D2HGDH protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-286 aa of BC036604 Sequence: MLPRRPLAWPAWLLRGAPGAAGSWGRPVGPLARRGCCSAPGTPEVPLTRERYPVQRLPFSTVSKQDLAAFERIVPGGVVTDPEALQAPNVDWLRTLRGCSKVLLRPRTSEEVSHILRHCHERNLAVNPQGGNTGMVGGSVPVFDEIILSTARMNRVLSFHSVSGILVCQAGCVLEELSRYVEERDFIMPLDLGAKGSCHIGGNVATNAGGLRFLRYGSLHGTVLGLEVVLADGTVLDCLTSLRKDNTGYDLKQLFIGSEGTLGIITTVSILCPPKPRAVNVAFLGC Predict reactive species
Full Name	D-2-hydroxyglutarate dehydrogenase
Calculated Molecular Weight	521 aa, 56 kDa
Observed Molecular Weight	56 kDa
GenBank Accession Number	BC036604
Gene Symbol	D2HGDH
Gene ID (NCBI)	728294
RRID	AB_2881744
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q8N465
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

D2HGDH(D-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as D2HGD and belongs to the FAD-binding oxidoreductase/transferase type 4 family.It catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.Defects in D2HGDH are the cause of D-2-hydroxyglutaric aciduria type 1 (D2HGA1).

Protocols

Product Specific Protocols
FC protocol for D2HGDH antibody 66364-1-Ig	Download protocol
IF protocol for D2HGDH antibody 66364-1-Ig	Download protocol
IHC protocol for D2HGDH antibody 66364-1-Ig	Download protocol
WB protocol for D2HGDH antibody 66364-1-Ig	Download protocol