DGKA AntibodyRabbit Polyclonal
|Tested applications||ELISA, WB, IHC, IP|
|Species specificity||human, mouse, rat; other species not tested.|
|Positive WB detected in||human brain tissue, HEK-293 cells, HeLa cells, human cerebellum tissue, Jurkat cells|
|Positive IHC detected in||human gliomas|
|Recommended dilution||WB: 1:500-1:5000|
IP = Immunoprecipitation
- IP result of DGKA antibody (11547-1-AP for IP and Detection) with Jurkat cell lysate.
- human brain tissue were subjected to SDS PAGE followed by western blot with 11547-1-AP(DGKA Antibody) at dilution of 1:1200
- Immunohistochemistry of paraffin-embedded human gliomas using 11547-1-AP(DGKA Antibody) at Dilution 1:100 (under 10x lens)
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||DGKA fusion protein ag2075||Full name||diacylglycerol kinase, alpha 80kDa|
|BC023523||Gene ID (NCBI)||1606|
|Gene symbol||DGKA||Synonyms||DAGK; DAGK1; DGK-alpha; MGC12821; MGC42356|
DGKA(Diacylglycerol kinase alpha) is also named as DAGK, DAGK1 and belongs to the eukaryotic diacylglycerol kinase family,which is suggested to attenuate diacylglycerol-induced cell responses through the phosphorylation of this second messenger to phosphatidic acid.DGKA regulates the secretion of lethal exosomes bearing Fas ligand during activation-induced cell death of T lymphocytes.This antibody is specific to DGKA.