DGKA Antibody
Rabbit Polyclonal
Catalog number: 11547-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IP |
| Cited applications | WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive WB detected in | human brain tissue, HEK-293 cells, HeLa cells, human cerebellum tissue, Jurkat cells |
| Positive IHC detected in | human gliomas |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP result of DGKA antibody (11547-1-AP for IP and Detection) with Jurkat cell lysate.
human brain tissue were subjected to SDS PAGE followed by western blot with 11547-1-AP(DGKA Antibody) at dilution of 1:1200
Immunohistochemistry of paraffin-embedded human gliomas using 11547-1-AP(DGKA Antibody) at Dilution 1:100 (under 10x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | DGKA fusion protein ag2075 | Full name | diacylglycerol kinase, alpha 80kDa |
|
Calculated molecular weight |
735aa,83kd |
Observed molecular
weight |
83kd |
|
GenBank accession number |
BC023523 | Gene ID (NCBI) | 1606 |
| Gene symbol | DGKA | Synonyms | DAGK; DAGK1; DGK-alpha; MGC12821; MGC42356 |
Background
DGKA(Diacylglycerol kinase alpha) is also named as DAGK, DAGK1 and belongs to the eukaryotic diacylglycerol kinase family,which is suggested to attenuate diacylglycerol-induced cell responses through the phosphorylation of this second messenger to phosphatidic acid.DGKA regulates the secretion of lethal exosomes bearing Fas ligand during activation-induced cell death of T lymphocytes.This antibody is specific to DGKA. |
