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ENPEP Polyclonal antibody

ENPEP Polyclonal Antibody for FC, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 17655-1-AP

Synonyms

Aminopeptidase A, AP A, APA, CD249, Differentiation antigen gp160, EAP, ENPEP, Glutamyl aminopeptidase, gp160

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ENPEP Polyclonal antibody (17655-1-AP)

    IF Staining of HepG2 using 17655-1-AP

    Immunofluorescent analysis of HepG2 cells, using ENPEP antibody 17655-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HepG2 cells using ENPEP Polyclonal antibody (17655-1-AP)

    FC experiment of HepG2 using 17655-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug ENPEP antibody (17655-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17655-1-AP targets ENPEP in IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ENPEP fusion protein Ag11903
Full Name glutamyl aminopeptidase (aminopeptidase A)
Calculated Molecular Weight 957 aa, 109 kDa
GenBank Accession NumberBC094770
Gene Symbol ENPEP
Gene ID (NCBI) 2028
RRIDAB_2099487
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for ENPEP antibody 17655-1-APDownload protocol
FC protocol for ENPEP antibody 17655-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
FC

Small

Engineering of the fluorescent-energy-conversion arm of phi29 DNA packaging motor for single-molecule studies.

Authors - Tae Jin Lee
View Article
mouseIF

Cell Rep

Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics.

Authors - Taha A Jan
View Article