EXOSC1 Polyclonal antibody

EXOSC1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12585-1-AP

Synonyms

CGI 108, CSL4, Csl4p, EXOSC1, exosome component 1, hCsl4p, p13, SKI4, Ski4p



Tested Applications

Positive WB detected inSGC-7901 cells, HeLa cells, human stomach tissue, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

12585-1-AP targets EXOSC1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, yeast
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXOSC1 fusion protein Ag3286
Full Name exosome component 1
Calculated Molecular Weight 195 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC022067
Gene Symbol EXOSC1
Gene ID (NCBI) 51013
RRIDAB_2101714
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXOSC1 is a core component of the exosome. The mammalian exosome is required for rapid degradation of AU rich element-containing RNAs but not for poly(A) shortening.

Protocols

Product Specific Protocols
WB protocol for EXOSC1 antibody 12585-1-APDownload protocol
IHC protocol for EXOSC1 antibody 12585-1-APDownload protocol
IP protocol for EXOSC1 antibody 12585-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
yeastWB

Am J Med Genet A

Pontocerebellar hypoplasia associated with p.Arg183Trp homozygous variant in EXOSC1 gene: A case report

Authors - Nadirah S Damseh
humanWB

Life Sci Alliance

Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication

Authors - Tobias Moll

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tsimafei (Verified Customer) (12-17-2023)

Nice antibody!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: mESCs
EXOSC1 Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:12585-1-AP)
FH

Andrea (Verified Customer) (01-11-2023)

Great antibody! Were incubated overnight at 4C

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mESCs
EXOSC1 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:12585-1-AP)