Filter: AllWBIHCIF/ICC

WB analysis using 24470-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24470-1-AP (EXOSC9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 24470-1-AP
WB result of EXOSC9 antibody (24470-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC9 transfected HeLa cells.
WB analysis of MCF-7 using 24470-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 24470-1-AP (EXOSC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human prostate hyperplasia using 24470-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24470-1-AP (EXOSC9 antibody) at dilution of 1:200 (under 40x lens).
IHC staining of mouse kidney using 24470-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24470-1-AP (EXOSC9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 24470-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EXOSC9 antibody (24470-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

EXOSC9 Polyclonal antibody

Name: EXOSC9 Polyclonal antibody
Brand: Proteintech
SKU: 24470-1-AP
Rating: 5.00 (2 reviews)

EXOSC9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 24470-1-AP

Publications(3)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PMSCL1, PM/Scl-75, P75 polymyositis-scleroderma overlap syndrome-associated autoantigen, Exosome complex component RRP45

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (3)
Protocols
Reviews (2)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, MCF-7 cells, HeLa cells, HepG2 cells, NIH/3T3 cells
Positive IHC detected in	human prostate hyperplasia tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IF	See 1 publications below

Product Information

24470-1-AP targets EXOSC9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, yeast
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag19768 Product name: Recombinant human EXOSC9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC142978 Sequence: MKETPLSNCERRFLLRAIEEKKRLDGRQTYDYRNIRISFGTDYGCCIVELGKTRVLGQVSCELVSPKLNRATEGILFFNLELSQMAAPAFEPGRQSDLLVKLNRLMERCLRNSKCIDTESLCVVAGEKVWQIRVDLHLLNHDGNIIDAASIAAIVALCHFRRPDVSVQGDEVTLYTPEERDPVPLSIHHMPICVSFAFFQQGAYLLVDPNEREERVMDGLLVIAMNKHREICTIQSSGGIMLLKDQVLRCSKIAGVKVAEITELILKALENDQKVRKEGGKFGFAESIANQRITAFKMEKAPIDTSDVEEKAEEIIAEAEPPSEVVSTPVLWTPGTAQIGEGVENSWGDLE Predict reactive species
Full Name	exosome component 9
Calculated Molecular Weight	456 aa, 51 kDa
Observed Molecular Weight	65-70 kDa
GenBank Accession Number	BC142978
Gene Symbol	EXOSC9
Gene ID (NCBI)	5393
RRID	AB_2879559
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q06265
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXOSC9, also named as PMSCL1 and PM/Scl-75, is a key subunit of the exosome complex. EXOSC9 maintains self-renewal in a cell-autonomous manner. EXOSC9 has 4 isoforms with MW 49 kDa, 51 kDa, 39 kDa and 41 kDa. It's polymyositis/scleroderma auto-antigen 75 kDa. The MW of EXOSC9 is migrated to 60-75 kDa for modification in WB detection.

Protocols

Product Specific Protocols
IF protocol for EXOSC9 antibody 24470-1-AP	Download protocol
IHC protocol for EXOSC9 antibody 24470-1-AP	Download protocol
WB protocol for EXOSC9 antibody 24470-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Life Sci Alliance Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication Authors - Tobias Moll View Article
yeast	WB	G3 (Bethesda) Humanized Saccharomyces cerevisiae provides a facile and effective tool to identify damaging human variants that cause exosomopathies Authors - Khondakar Sayef Ahammed View Article
human	IF	Mol Cell Degradation of circular RNA by the ribonuclease DIS3 Authors - Xiao Tao View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH Tsimafei (Verified Customer) (12-17-2023)	Great antibody Applications: Western Blot Primary Antibody Dilution: 1:3000 Cell Tissue Type: mESCs EXOSC9 Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:24470-1-AP)
FH Nikolaus (Verified Customer) (07-14-2022)	Antibodies were incubated ON at 4C. Exosc9 depletion was used as a control to check the specificity of the band. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: mESCs EXOSC9 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:24470-1-AP)