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  • Featured Product
  • KD/KO Validated

EXOSC9 Polyclonal antibody

EXOSC9 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24470-1-AP

Synonyms

Autoantigen PM/Scl 1, EXOSC9, exosome component 9, p5, p6, PM/Scl 75, PMSCL1, RRP45, Rrp45p

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EXOSC9 Polyclonal antibody (24470-1-AP)

    WB analysis using 24470-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24470-1-AP (EXOSC9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EXOSC9 Polyclonal antibody (24470-1-AP)

    WB analysis of HeLa using 24470-1-AP

    WB result of EXOSC9 antibody (24470-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC9 transfected HeLa cells.

  • Western Blot (WB) analysis of MCF-7 cells using EXOSC9 Polyclonal antibody (24470-1-AP)

    WB analysis of MCF-7 using 24470-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24470-1-AP (EXOSC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using EXOSC9 Polyclonal antibody (24470-1-AP)

    IHC staining of human prostate hyperplasia using 24470-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24470-1-AP (EXOSC9 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using EXOSC9 Polyclonal antibody (24470-1-AP)

    IHC staining of mouse kidney using 24470-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24470-1-AP (EXOSC9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EXOSC9 Polyclonal antibody (24470-1-AP)

    IF Staining of HeLa using 24470-1-AP

    Immunofluorescent analysis of HeLa cells using 24470-1-AP (EXOSC9 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, HeLa cells, HepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman prostate hyperplasia tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

24470-1-AP targets EXOSC9 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXOSC9 fusion protein Ag19768
Full Name exosome component 9
Calculated Molecular Weight 456 aa, 51 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC142978
Gene Symbol EXOSC9
Gene ID (NCBI) 5393
RRIDAB_2879559
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXOSC9, also named as PMSCL1 and PM/Scl-75, is a key subunit of the exosome complex. EXOSC9 maintains self-renewal in a cell-autonomous manner. EXOSC9 has 4 isoforms with MW 49 kDa, 51 kDa, 39 kDa and 41 kDa. It's polymyositis/scleroderma auto-antigen 75 kDa. The MW of EXOSC9 is migrated to 60-75 kDa for modification in WB detection.

Protocols

Product Specific Protocols
WB protocol for EXOSC9 antibody 24470-1-APDownload protocol
IHC protocol for EXOSC9 antibody 24470-1-APDownload protocol
IF protocol for EXOSC9 antibody 24470-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Life Sci Alliance

Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication

Authors - Tobias Moll
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tsimafei (Verified Customer) (12-17-2023)

Great antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: mESCs
EXOSC9 Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:24470-1-AP)
FH

Nikolaus (Verified Customer) (07-14-2022)

Antibodies were incubated ON at 4C. Exosc9 depletion was used as a control to check the specificity of the band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mESCs
EXOSC9 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:24470-1-AP)