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FAM175A Polyclonal antibody

FAM175A Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 14366-1-AP

Synonyms

ABRA1, CCDC98, FAM175A, Protein FAM175A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM175A Polyclonal antibody (14366-1-AP)

    WB analysis using 14366-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 14366-1-AP (FAM175A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using FAM175A Polyclonal antibody (14366-1-AP)

    WB analysis of human testis using 14366-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 14366-1-AP (FAM175A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using FAM175A Polyclonal antibody (14366-1-AP)

    WB analysis of Raji using 14366-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 14366-1-AP (FAM175A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using FAM175A Polyclonal antibody (14366-1-AP)

    WB analysis of MCF-7 using 14366-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 14366-1-AP (FAM175A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using FAM175A Polyclonal antibody (14366-1-AP)

    IF Staining of MCF-7 using 14366-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 14366-1-AP (FAM175A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293T cells, MCF-7 cells, human testis tissue, Raji cells, Hela cells, Jurkat cells
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14366-1-AP targets FAM175A in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM175A fusion protein Ag5688
Full Name family with sequence similarity 175, member A
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC039573
Gene Symbol FAM175A
Gene ID (NCBI) 84142
RRIDAB_2262501
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The family with sequence similarity 175 member A gene (FAM175A; also known as ABRAXAS1, CCDC98 and ABRA1), a member of the DNA repair family, contributes to the BRCA1 (BRCA1 DNA repair associated)-dependent DNA damage response and is associated with age at natural menopause.

Protocols

Product Specific Protocols
WB protocol for FAM175A antibody 14366-1-APDownload protocol
IF protocol for FAM175A antibody 14366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

RAP80 phase separation at DNA double-strand break promotes BRCA1 recruitment

Authors - Caolitao Qin
View Article