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  • KD/KO Validated

Kindlin 1 Polyclonal antibody

Kindlin 1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22215-1-AP

Synonyms

C20orf42, DTGCU2, Fermitin family homolog 1, FERMT1, KIND1, Kindlerin, Kindlin 1, Kindlin syndrome protein, Unc 112 related protein 1, UNC112A, URP1



Tested Applications

Positive WB detected inmouse kidney tissue, COLO 320 cells, HEK-293 cells
Positive IHC detected inhuman pancreas tissue, human colon cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22215-1-AP targets Kindlin 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kindlin 1 fusion protein Ag17543
Full Name fermitin family homolog 1 (Drosophila)
Calculated Molecular Weight 677 aa, 77 kDa
Observed Molecular Weight 70-77 kDa
GenBank Accession NumberBC035882
Gene Symbol FERMT1
Gene ID (NCBI) 55612
RRIDAB_2879033
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kindlin-1 is a FERM domain containing adaptor protein that is found predominantly at cell-extracellular matrix adhesions where it binds to β-integrin subunits and is required for integrin activation. Loss of function mutations in the FERMT1 gene which encodes Kindlin-1 leads to the development of Kindler Syndrome (KS) an autosomal recessive skin disorder characterized by skin blistering, photosensitivity, and predisposition to aggressive squamous cell carcinoma (SCC).

Protocols

Product Specific Protocols
WB protocol for Kindlin 1 antibody 22215-1-APDownload protocol
IHC protocol for Kindlin 1 antibody 22215-1-APDownload protocol
IF protocol for Kindlin 1 antibody 22215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cancer Cell Int

FERMT1 contributes to the migration and invasion of nasopharyngeal carcinoma through epithelial-mesenchymal transition and cell cycle arrest.

Authors - Lingling Li
  • KD Validated
ratWB

Eur J Neurosci

Wnt10a/β-catenin signaling is involved in kindlin-1-mediated astrocyte activation in a chronic construction injury rat model.

Authors - Baisong Zhao
mouseIHC,IF

Int J Mol Med

Kindlin-1 contributes to EGF-induced re-epithelialization in skin wound healing.

Authors - Congcong Shen
mouse,ratWB,IF

Exp Anim

FERMT1 knockdown attenuates inflammation induced by intracerebral hemorrhage in rats via NLRP3/NF-κB pathway

Authors - Jianqiang Wei
  • KD Validated
mouseWB

Cell Rep

Distinct bidirectional regulation of LFA1 and α4β7 by Rap1 and integrin adaptors in T cells under shear flow

Authors - Yuji Kamioka