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  • KD/KO Validated

FXR1 Polyclonal antibody

FXR1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13194-1-AP

Synonyms

FXR1, hFXR1p



Tested Applications

Positive WB detected inHEK-293 cells, mouse heart tissue, NIH/3T3 cells
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inEthacrynic acid treated HepG2 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13194-1-AP targets FXR1 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FXR1 fusion protein Ag3873
Full Name fragile X mental retardation, autosomal homolog 1
Calculated Molecular Weight 621 aa, 70 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberBC028983
Gene Symbol FXR1
Gene ID (NCBI) 8087
RRIDAB_2110702
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tumour necrosis factor-alpha (TNF-alpha) is a key mediator of inflammation in host defence against infection and in autoimmune disease. Its production is controlled post-transcriptionally by multiple RNA-binding proteins that interact with the TNF-alpha AU-rich element and regulate its expression; Fragile X mental retardation-related protein 1 (FXR1) is one of these. FXR1 (fragile-X-mental retardation-related protein 1) are RNA-binding proteins that have been demonstrated to impact miRNA-mediated, post-transcriptional gene regulation, and required for embryonic and postnatal development of muscle tissue. It can regulate intracellular transport and local translation of certain mRNAs. The FXR1 exists some isoforms with the MW 69 kDa and 60 kDa.

Protocols

Product Specific Protocols
WB protocol for FXR1 antibody 13194-1-APDownload protocol
IHC protocol for FXR1 antibody 13194-1-APDownload protocol
IF protocol for FXR1 antibody 13194-1-APDownload protocol
IP protocol for FXR1 antibody 13194-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell

Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.

Authors - David W Sanders
mouseIF

Neuron

Fmr1 KO and fenobam treatment differentially impact distinct synapse populations of mouse neocortex.

Authors - Gordon X Wang
mouseIF

Nat Commun

Microtubule-based transport is essential to distribute RNA and nascent protein in skeletal muscle

Authors - Lance T. Denes

Elife

Sub-synaptic, multiplexed analysis of proteins reveals Fragile X related protein 2 is mislocalized in Fmr1 KO synapses.

Authors - Gordon X Wang
humanRIP

Elife

The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus.

Authors - Belinda Baquero-Perez
mouseWB

J Neurosci

Dysregulated RNA-Induced Silencing Complex (RISC) Assembly within CNS Corresponds with Abnormal miRNA Expression during Autoimmune Demyelination.

Authors - Przemysław Lewkowicz

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Chao (Verified Customer) (01-30-2020)

Western blot show strong band in the basal state.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart